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Literature summary for 3.2.1.37 extracted from
- Characteristics of Trichoderma reesei beta-xylosidase and its use in the hydrolysis of solubilized xylans (1988), Appl. Microbiol. Biotechnol., 28, 425-432.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| beta-D-xylose | 25% competitive inhibition at 50 mM | Trichoderma reesei |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Trichoderma reesei | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 100000 | - |
SDS-PAGE | Trichoderma reesei |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| xylan + H2O | Trichoderma reesei | - |
beta-D-xylose + ? | - |
? | |
| xylo-oligosaccharides + H2O | Trichoderma reesei | - |
beta-D-xylose + ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trichoderma reesei | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | 7.5% carbohydrate moiety | Trichoderma reesei |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity by 2step chromatography | Trichoderma reesei |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | - |
Trichoderma reesei | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| p-nitrophenyl alpha-L-arabinofuranoside + H2O | low affinity, 70% activity of that for p-nitrophenyl beta-D-xylopyranose | Trichoderma reesei | p-nitrophenol + alpha-L-arabinofuranose | - |
? | |
| p-nitrophenyl beta-D-xylopyranoside + H2O | - |
Trichoderma reesei | p-nitrophenol + beta-D-xylopyranose | - |
? | |
| xylan + H2O | - |
Trichoderma reesei | beta-D-xylose + ? | - |
? | |
| xylo-oligosaccharides + H2O | - |
Trichoderma reesei | beta-D-xylose + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 100000, SDS-PAGE | Trichoderma reesei |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Trichoderma reesei |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
stable up to | Trichoderma reesei |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
- |
Trichoderma reesei |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 6 | stable for 24 h at 45°C | Trichoderma reesei |
| 6 | - |
50% activity | Trichoderma reesei |
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