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Literature summary for 3.2.1.37 extracted from

  • John, M.; Schmidt, J.
    Xylanases and beta-xylosidase of Trichoderma lignorum (1988), Methods Enzymol., 160, 662-671.
No PubMed abstract available
Search for more literature for 3.2.1.37

Inhibitors

Inhibitors Comment Organism Structure
beta-D-xylose 60% inhibition at 10 mM Trichoderma viride
glucose 16% inhibition at 10 mM Trichoderma viride
Hg2+ strong inhibition at 0.1 mM Trichoderma viride
methyl-beta-D-xylopyranoside 34% inhibition at 10 mM Trichoderma viride

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.08
-
 p-nitrophenyl beta-D-xylopyranoside
-
 Trichoderma viride
5
-
 p-nitrophenyl beta-D-xylopyranoside
-
 Trichoderma viride

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Trichoderma viride
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ stimulation at 1 mM Trichoderma viride
Mn2+ stimulation at 1 mM Trichoderma viride

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 2 * 50000, SDS-PAGE Trichoderma viride
100000
-
 gel filtration Trichoderma viride

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
xylan + H2O Trichoderma viride limited capability to produce xylose due to synergistic action of xylanase and beta-xylosidase, synergistic action also needed for libration of acidic substituents beta-D-xylose + ?
-
 ?
xylo-oligosaccharides + H2O Trichoderma viride smaller xylooligosaccharides from 2-5mers less rapidly hydrolyzed beta-D-xylose + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Trichoderma viride
-
 three isoforms
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein 25% carbohydrate moiety Trichoderma viride

Purification (Commentary)

Purification (Comment) Organism
to homogeneity by 2step chromatography Trichoderma viride

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 Trichoderma viride
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4
-
-
 Trichoderma viride
28.2
-
-
 Trichoderma viride

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
p-nitrophenyl beta-D-xylopyranoside + H2O
-
 Trichoderma viride p-nitrophenol + beta-D-xylopyranose
-
 ?
xylan + H2O limited capability to produce xylose due to synergistic action of xylanase and beta-xylosidase, synergistic action also needed for libration of acidic substituents Trichoderma viride beta-D-xylose + ?
-
 ?
xylo-oligosaccharides + H2O smaller xylooligosaccharides from 2-5mers less rapidly hydrolyzed Trichoderma viride beta-D-xylose + ?
-
 ?
xyloheptaose + H2O
-
 Trichoderma viride beta-D-xylose + ?
-
 ?
xylohexaose + H2O
-
 Trichoderma viride beta-D-xylose + ?
-
 ?
xylooctaose + H2O
-
 Trichoderma viride beta-D-xylose + ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 50000, SDS-PAGE Trichoderma viride

Synonyms

Synonyms Comment Organism
beta-D-xyloside xylohydrolase
-
 Trichoderma viride

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
 Trichoderma viride

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 70% activity after 1 h Trichoderma viride
80
-
 rapidly inactivated above Trichoderma viride

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
-
 Trichoderma viride

pH Stability

pH Stability pH Stability Maximum Comment Organism
3.5 7.5
-
 Trichoderma viride