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Literature summary for 3.2.1.37 extracted from

  • Matsuo, M.; Yasui, T.
    beta-Xylosidases of several fungi (1988), Methods Enzymol., 160, 684-694.
No PubMed abstract available

General Stability

General Stability Organism
addition of Ca2+ protects against thermal inactivation Malbranchea pulchella var. sulfurea

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ strong inhibition Malbranchea pulchella var. sulfurea
Hg2+ complete inhibition Aspergillus nidulans
Hg2+ strong inhibition Malbranchea pulchella var. sulfurea
Hg2+ strong inhibition Trichoderma viride
N-bromosuccinimide complete inhibition at 1 mM Aspergillus nidulans
N-bromosuccinimide strong inhibition Malbranchea pulchella var. sulfurea
N-bromosuccinimide strong inhibition Trichoderma viride
p-chloromercuribenzoate strong inhibition Aspergillus nidulans
p-chloromercuribenzoate strong inhibition Malbranchea pulchella var. sulfurea
SDS complete inhibition Aspergillus nidulans
SDS strong inhibition Malbranchea pulchella var. sulfurea
Zn2+ strong inhibition Malbranchea pulchella var. sulfurea

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Aspergillus nidulans
-
-
extracellular
-
Trichoderma viride
-
-
extracellular
-
Malbranchea pulchella var. sulfurea
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26000
-
gel filtration Malbranchea pulchella var. sulfurea
101000 102000 SDS-PAGE, gel filtration Trichoderma viride
116000
-
2 * 116000, SDS-PAGE Aspergillus nidulans
240000
-
gel filtration, SDS-PAGE Aspergillus nidulans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
xylan + H2O Malbranchea pulchella var. sulfurea
-
beta-D-xylose + ?
-
?
xylobiose + H2O Aspergillus nidulans
-
beta-D-xylose + D-xylose
-
?
xylobiose + H2O Trichoderma viride
-
beta-D-xylose + D-xylose
-
?
xylobiose + H2O Malbranchea pulchella var. sulfurea
-
beta-D-xylose + D-xylose
-
?

Organism

Organism UniProt Comment Textmining
Aspergillus nidulans
-
-
-
Malbranchea pulchella var. sulfurea
-
-
-
Trichoderma viride
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein 4% carbohydrate moiety Aspergillus nidulans
glycoprotein 4.5% carbohydrate moiety Trichoderma viride

Purification (Commentary)

Purification (Comment) Organism
to homogeneity by chromatography and electrophoretic steps Malbranchea pulchella var. sulfurea
to homogeneity by chromatography and isoelectric focusing Trichoderma viride
to homogeneity by chromatography, electrofocusing Aspergillus nidulans

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
Aspergillus nidulans
-
cell culture
-
Trichoderma viride
-
cell culture
-
Malbranchea pulchella var. sulfurea
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
10.5
-
-
Malbranchea pulchella var. sulfurea
10.8
-
-
Trichoderma viride
62.9
-
-
Aspergillus nidulans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzyl-beta-D-xyloside + H2O low activity Aspergillus nidulans phenol + beta-D-xylose
-
?
butyl-beta-D-xyloside low activity Aspergillus nidulans butanol + beta-D-xylose
-
?
butyl-beta-D-xyloside low activity Trichoderma viride butanol + beta-D-xylose
-
?
cyclohexyl-beta-D-xyloside + H2O low activity Aspergillus nidulans cyclohexanol + beta-D-xylose
-
?
cyclohexyl-beta-D-xyloside + H2O low activity Trichoderma viride cyclohexanol + beta-D-xylose
-
?
methyl-beta-D-xyloside + H2O very low activity Trichoderma viride beta-D-xylose + methanol
-
?
methyl-beta-D-xyloside + H2O very low activity Trichoderma viride ?
-
?
o-nitrophenyl-beta-D-xyloside + H2O
-
Aspergillus nidulans o-nitrophenol + beta-D-xylose
-
?
o-nitrophenyl-beta-D-xyloside + H2O
-
Trichoderma viride o-nitrophenol + beta-D-xylose
-
?
p-nitrophenyl beta-D-xyloside + H2O
-
Aspergillus nidulans p-nitrophenol + beta-D-xylose
-
?
p-nitrophenyl beta-D-xyloside + H2O
-
Trichoderma viride p-nitrophenol + beta-D-xylose
-
?
p-nitrophenyl beta-D-xyloside + H2O
-
Malbranchea pulchella var. sulfurea p-nitrophenol + beta-D-xylose
-
?
p-nitrophenyl-beta-D-xyloside + H2O beta-xylosidase-1 Malbranchea pulchella var. sulfurea p-nitrophenol + alpha-D-xylose inversion of anomeric configuration ?
xylan + H2O
-
Malbranchea pulchella var. sulfurea beta-D-xylose + ?
-
?
xylobiose + H2O
-
Aspergillus nidulans beta-D-xylose + D-xylose
-
?
xylobiose + H2O
-
Trichoderma viride beta-D-xylose + D-xylose
-
?
xylobiose + H2O
-
Malbranchea pulchella var. sulfurea beta-D-xylose + D-xylose
-
?

Subunits

Subunits Comment Organism
dimer 2 * 116000, SDS-PAGE Aspergillus nidulans
monomer 1 * 101000-102000, SDS-PAGE, gel filtration Trichoderma viride
monomer 1 * 26000, SDS-PAGE, gel filtration Malbranchea pulchella var. sulfurea

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Malbranchea pulchella var. sulfurea
55
-
-
Aspergillus nidulans
55
-
-
Trichoderma viride

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
stable up to Aspergillus nidulans
50
-
50% activity retained after 15 min Malbranchea pulchella var. sulfurea
55
-
stable up to Trichoderma viride

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5
-
-
Trichoderma viride
4.5 5
-
Aspergillus nidulans
6.2 6.8
-
Malbranchea pulchella var. sulfurea

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 4 stable for 30 min at 50°C Trichoderma viride
4.5 5 stable for 30 min at 50°C Aspergillus nidulans
6.3 6.7 stable within Malbranchea pulchella var. sulfurea