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Literature summary for 3.2.1.37 extracted from

  • Nanmori, T.; Watanabe, T.; Shinke, R.; Kohno, A.; Kawamura, Y.
    Purification and properties of thermostable xylanase and beta-xylosidase produced by a newly isolated Bacillus stearothermophilus strain (1990), J. Bacteriol., 172, 6669-6672.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.37

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2
-
 p-nitrophenyl beta-D-xyloside
-
 Geobacillus stearothermophilus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Geobacillus stearothermophilus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
75000
-
 2 * 75000, SDS-PAGE Geobacillus stearothermophilus
150000
-
 gel filtration, SDS-PAGE Geobacillus stearothermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
xylan + H2O Geobacillus stearothermophilus complete degration of xylan requires synergistic action of two types of enzyme, endo-1,4-xylanase to form xylooligosaccharides and beta-D-xylosidase, which hydrolyses xylooligosaccharides to xylose beta-D-xylose + ?
-
 ?
xylo-oligosaccharides + H2O Geobacillus stearothermophilus derived from xylan beta-D-xylose + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity by 4step chromatography Geobacillus stearothermophilus

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 Geobacillus stearothermophilus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
34.2
-
-
 Geobacillus stearothermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
p-nitrophenyl beta-D-xyloside + H2O
-
 Geobacillus stearothermophilus p-nitrophenol + beta-D-xylose
-
 ?
xylan + H2O complete degration of xylan requires synergistic action of two types of enzyme, endo-1,4-xylanase to form xylooligosaccharides and beta-D-xylosidase, which hydrolyses xylooligosaccharides to xylose Geobacillus stearothermophilus beta-D-xylose + ?
-
 ?
xylo-oligosaccharides + H2O derived from xylan Geobacillus stearothermophilus beta-D-xylose + ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 75000, SDS-PAGE Geobacillus stearothermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
 Geobacillus stearothermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 stable for 1 h Geobacillus stearothermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Geobacillus stearothermophilus

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 8 stable within Geobacillus stearothermophilus