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Literature summary for 3.2.1.37 extracted from
- Purification and characterization of an extracellular beta-xylosidase from rumen anaerobic fungus Neocallimastix frontalis (1990), FEMS Microbiol. Lett., 72, 11-16.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | 50% inhibition | Neocallimastix frontalis |  |
| Hg2+ | complete inhibition | Neocallimastix frontalis | |
| p-chloromercuribenzoate | complete inhibition | Neocallimastix frontalis | |
| SDS | complete inhibition | Neocallimastix frontalis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 122 | - |
p-nitrophenyl beta-D-xylopyranoside | - |
Neocallimastix frontalis | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Neocallimastix frontalis | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Neocallimastix frontalis | |
| Co2+ | - |
Neocallimastix frontalis | |
| Mg2+ | - |
Neocallimastix frontalis | |
| Mn2+ | considerable increase of activity | Neocallimastix frontalis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 90000 | - |
2 * 90000, SDS-PAGE | Neocallimastix frontalis |
| 180000 | - |
gel filtration, SDS-PAGE | Neocallimastix frontalis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beta-1,4-xylan + H2O | Neocallimastix frontalis | - |
beta-D-xylose + ? | - |
? | |
| xylan + H2O | Neocallimastix frontalis | complete degration of xylan requires synergistic action of two types of enzyme, endo-1,4-xylanase to form xylooligosaccharides and beta-D-xylosidase, which hydrolyses xylooligosaccharides to xylose | beta-D-xylose + ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Neocallimastix frontalis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity by 2step chromatography and isoelectric focusing | Neocallimastix frontalis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| zoospore | zoospore culture, induction of enzyme production highest in cellubiose/xylan medium | Neocallimastix frontalis | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 4.3 | - |
- |
Neocallimastix frontalis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beta-1,4-xylan + H2O | - |
Neocallimastix frontalis | beta-D-xylose + ? | - |
? | |
| p-nitrophenyl beta-D-xylopyranoside + H2O | - |
Neocallimastix frontalis | p-nitrophenol + beta-D-xylopyranose | - |
? | |
| xylan + H2O | complete degration of xylan requires synergistic action of two types of enzyme, endo-1,4-xylanase to form xylooligosaccharides and beta-D-xylosidase, which hydrolyses xylooligosaccharides to xylose | Neocallimastix frontalis | beta-D-xylose + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 90000, SDS-PAGE | Neocallimastix frontalis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
- |
Neocallimastix frontalis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
rapid decrease of activity above and below | Neocallimastix frontalis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 6.5 | - |
Neocallimastix frontalis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 6.5 | stable during 1 h at 20°C | Neocallimastix frontalis |
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