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Literature summary for 3.2.1.37 extracted from
- Purification and some properties of a beta-xylosidase and an alpha-fucosidase from apple snails (Pomacea canaliculata) (1996), Biosci. Biotechnol. Biochem., 60, 249-254.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| p-chloromercuribenzoate | 29% inhibition at 1 mM | Pomacea canaliculata |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 85000 | 96000 | SDS-PAGE, sedimentation coefficient | Pomacea canaliculata |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beta-1,3-xylan + H2O | Pomacea canaliculata | complete hydrolysis after prolonged incubation | beta-D-xylose + ? | - |
? | |
| beta-1,4-xylan + H2O | Pomacea canaliculata | complete hydrolysis after prolonged incubation | beta-D-xylose + ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pomacea canaliculata | - |
apple snail | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | 4.6% sugar content | Pomacea canaliculata |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity by 4step chromatography techniques | Pomacea canaliculata |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| viscus | - |
Pomacea canaliculata | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.95 | - |
- |
Pomacea canaliculata |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beta-1,3-xylan + H2O | complete hydrolysis after prolonged incubation | Pomacea canaliculata | beta-D-xylose + ? | - |
? | |
| beta-1,4-xylan + H2O | complete hydrolysis after prolonged incubation | Pomacea canaliculata | beta-D-xylose + ? | - |
? | |
| Manalpha(1-6)[Manalpha(1-3)Xylbeta(1-2)]Manbeta(1-4)GlcNAcbeta(1-4)[Fucalpha(1-3)]GlcNAc--pyridylaminoside + H2O | complete hydrolysis | Pomacea canaliculata | beta-D-xylose + Manalpha(1-6)[Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)[Fucalpha(1-3)]GlcNAc-pyridylaminoside | - |
? | |
| p-nitrophenyl beta-D-xyloside + H2O | - |
Pomacea canaliculata | p-nitrophenol + beta-D-xylose | - |
? | |
| Xylbeta(1-2)Manbeta(1-4)GlcNAcbeta(1-4)[Fucalpha(1-3)]GlcNAc-pyridylaminoside + H2O | complete hydrolysis | Pomacea canaliculata | beta-D-xylose + Manbeta(1-4)GlcNAcbeta(1-4)[Fucalpha(1-3)]GlcNAc-pyridylaminoside | - |
? | |
| [Manalpha(1-6)Manalpha(1-3)Xylbeta(1-2)]Manbeta(1-4)GlcNAcbeta(1-4)[Fucalpha(1-3)]GlcNAc-pyridylaminoside + H2O | 50% hydrolysis | Pomacea canaliculata | beta-D-xylose + [Manalpha(1-6)Manalpha(1-6)]Manbeta(1-4)GlcNAcbeta(1-4)[Fucalpha(1-3)]GlcNAc-pyridylaminoside | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 85000-96000, SDS-PAGE, sedimentation coefficient | Pomacea canaliculata |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
stable up to within 3 min | Pomacea canaliculata |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3.6 | - |
- |
Pomacea canaliculata |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 10 | unstable below pH 3, activity lost at pH 2 | Pomacea canaliculata |
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