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Literature summary for 3.2.1.35 extracted from

  • Vivas-Ruiz, D.E.; Gonzalez-Kozlova, E.E.; Delgadillo, J.; Palermo, P.M.; Sandoval, G.A.; Lazo, F.; Rodriguez, E.; Chavez-Olortegui, C.; Yarleque, A.; Sanchez, E.F.
    Biochemical and molecular characterization of the hyaluronidase from Bothrops atrox Peruvian snake venom (2019), Biochimie, 162, 33-45 .
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol
-
Bothrops atrox
Ba2+ 0.2 M, about 90% loss of activity Bothrops atrox
Br2+ 0.2 M, about 40% loss of activity Bothrops atrox
Cd2+ 0.2 M, complete loss of activity Bothrops atrox
Cu2+ 0.2 M, about 90% loss of activity Bothrops atrox
dexamethasone
-
Bothrops atrox
EDTA
-
Bothrops atrox
iodoacetate
-
Bothrops atrox
Mg2+ 0.2 M, about 20% loss of activity Bothrops atrox
N-alpha-tosyl-L-lysine chloromethyl ketone
-
Bothrops atrox
Sr2+ 0.2 M, about 40% loss of activity Bothrops atrox
Zn2+ 0.2 M, complete loss of activity Bothrops atrox

Metals/Ions

Metals/Ions Comment Organism Structure
K+ 0.2 M, activates to about 105% of control Bothrops atrox
Na+ 0.2 M, activates to about 120% of control Bothrops atrox

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
69000
-
SDS-PAGE Bothrops atrox

Organism

Organism UniProt Comment Textmining
Bothrops atrox A0A2H4Z8F4
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
Bothrops atrox

Purification (Commentary)

Purification (Comment) Organism
-
Bothrops atrox

Source Tissue

Source Tissue Comment Organism Textmining
venom
-
Bothrops atrox
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
hyaluronan + H2O
-
Bothrops atrox ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 69000, SDS-PAGE Bothrops atrox

Synonyms

Synonyms Comment Organism
Hyal-Ba
-
Bothrops atrox
hyaluronidase
-
Bothrops atrox

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Bothrops atrox

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 50 20°C: about 40% of maximal activity, 50°C: about 40% of maximal activity Bothrops atrox

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Bothrops atrox

pH Range

pH Minimum pH Maximum Comment Organism
4 7 pH 4.0: about 90% of maximal activity, pH 7.0: about 75% of maximal activity Bothrops atrox

pH Stability

pH Stability pH Stability Maximum Comment Organism
5
-
stable at pH 5.0 for 40 h, with a slight decrease after 160 h incubation Bothrops atrox

General Information

General Information Comment Organism
physiological function the enzyme potentiates the hemorrhagic and hemolytic activity of whole venom, but decreases subplantar edema caused by an L-amino acid oxidase Bothrops atrox