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Literature summary for 3.2.1.35 extracted from

  • Li, R.; Yu, Q.; Han, L.; Rong, L.; Yang, M.; An, M.
    Isolation and enzymatic characterization of the first reported hyaluronidase from Yak (Bos grunniens) testis (2014), Korean J. Chem. Engin., 31, 2027-2034.
No PubMed abstract available
Search for more literature for 3.2.1.35

General Stability

General Stability Organism
adding bovine serum albumin (1.0 mg/ml) to the enzyme sample prior to freezing results in complete retention of enzyme activity Bos grunniens

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 65% residual activity at 50 mM Bos grunniens
EDTA complete inhibition at 10 mM Bos grunniens
Fe2+ 77% residual activity at 50 mM Bos grunniens
Fe3+ 20% residual activity at 50 mM Bos grunniens
heparin 90% inhibition at 1.5 mg/ml Bos grunniens
Mg2+ 46% residual activity at 50 mM Bos grunniens
additional information not affected by ascorbic acid, phenylmethylsulfonyl fluoride, dithiothreitol, glutathione (reduced), or L-cysteine Bos grunniens
NaCl 0.5 M causes 92% inhibition Bos grunniens
Urea complete inhibition at 10 mM Bos grunniens

Metals/Ions

Metals/Ions Comment Organism Structure
additional information not affected by Cu2+, Zn2+, and Co2+ Bos grunniens
NaCl maximum activity in the presence of 0.15 M NaCl Bos grunniens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
 gel filtration Bos grunniens

Organism

Organism UniProt Comment Textmining
Bos grunniens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
SP Sepharose column chromatography and Sephacryl S-100 gel filtration Bos grunniens

Source Tissue

Source Tissue Comment Organism Textmining
testis
-
 Bos grunniens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.85
-
 crude enzyme, at pH 3.8 and 27°C Bos grunniens
20.4
-
 after 24fold purification, at pH 3.8 and 27°C Bos grunniens

Storage Stability

Storage Stability Organism
22°C, 100 mM sodium acetate buffer (pH 3.8) containing 150 mM NaCl, 4 weeks, 58% activity retained Bos grunniens
4°C, 100 mM sodium acetate buffer (pH 3.8) containing 150 mM NaCl, 4 weeks, 92% activity retained Bos grunniens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chondroitin 4-sulfate + H2O 28% activity compared to sodium hyaluronate Bos grunniens ?
-
 ?
chondroitin 6-sulfate + H2O 19% activity compared to sodium hyaluronate Bos grunniens ?
-
 ?
dermatan + H2O 7% activity compared to sodium hyaluronate Bos grunniens ?
-
 ?
sodium hyaluronate + H2O 100% activity Bos grunniens ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 55000, SDS-PAGE Bos grunniens

Synonyms

Synonyms Comment Organism
Hyal
-
 Bos grunniens
hyaluronidase
-
 Bos grunniens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Bos grunniens

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 55 more than 50% activity between 20 and 55°C. Activity is abolished at 60°C Bos grunniens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3.8
-
-
 Bos grunniens

pH Range

pH Minimum pH Maximum Comment Organism
2.5 9 the optimum activity at pH 3.8 decreases by half at pH 2.5 and 6.0 and is virtually abolished at pH 9.0 Bos grunniens