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Literature summary for 3.2.1.35 extracted from
- Isolation, enzymatic characterization and antiedematogenic activity of the first reported rattlesnake hyaluronidase from Crotalus durissus terrificus venom (2012), Biochimie, 94, 2740-2748.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | complete inhibition at 0.2 mM | Crotalus durissus terrificus |  |
| Mg2+ | complete inhibition at 0.2 mM | Crotalus durissus terrificus | |
| NaCl | 1 M NaCl significantly reduces the enzyme activity | Crotalus durissus terrificus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| NaCl | maximum activity in the presence of 0.2 M NaCl | Crotalus durissus terrificus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 64500 | - |
1 * 64500, SDS-PAGE | Crotalus durissus terrificus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| hyaluronan + 2 H2O | Crotalus durissus terrificus | highly specific substrate with 10fold higher activity compared to chondroitin 4-sulfate, chondroitin 6-sulfate, or dermatan | N-acetyl-beta-D-glucosamine + D-glucuronate + ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Crotalus durissus terrificus | B3EWP2 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Crotalus durissus terrificus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| CM-cellulose-52 column chromatography, phenyl-Sepharose column chromatography, and Sephacryl S-100 gel filtration | Crotalus durissus terrificus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| venom | - |
Crotalus durissus terrificus | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 7°C, purification buffer, 60 days, remains stable | Crotalus durissus terrificus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chondroitin 4-sulfate + H2O | - |
Crotalus durissus terrificus | ? | - |
? | |
| chondroitin 6-sulfate + H2O | - |
Crotalus durissus terrificus | ? | - |
? | |
| dermatan + H2O | - |
Crotalus durissus terrificus | ? | - |
? | |
| hyaluronan + 2 H2O | highly specific substrate with 10fold higher activity compared to chondroitin 4-sulfate, chondroitin 6-sulfate, or dermatan | Crotalus durissus terrificus | N-acetyl-beta-D-glucosamine + D-glucuronate + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 64500, SDS-PAGE | Crotalus durissus terrificus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Hya1 | - |
Crotalus durissus terrificus |
| hyaluronidase | - |
Crotalus durissus terrificus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Crotalus durissus terrificus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 50 | more than 50% activity between 25 and 50°C | Crotalus durissus terrificus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Crotalus durissus terrificus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 7 | about 40% activity at pH 4.0, more than 90% activity at pH 5.0-6.0, about 50% activity at pH 6.5, about 10% activity at pH 7.0 | Crotalus durissus terrificus |
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Release 2023.1 (January 2023)
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