Literature summary for 3.2.1.35 extracted from

Andre, B.; Duterme, C.; Van Moer, K.; Mertens-Strijthagen, J.; Jadot, M.; Flamion, B.
Hyal2 is a glycosylphosphatidylinositol-anchored, lipid raft-associated hyaluronidase (2011), Biochem. Biophys. Res. Commun., 411, 175-179.

Cloned(Commentary)

Cloned (Comment)	Organism
transfection of COS-7 cell	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	Hyal2 is strongly associated with cell membrane fractions from which it can be extracted using a Triton X-114 treatment, but not an osmotic shock or an alkaline carbonate solution. Treatment of membrane preparations with phosphatidylinositol-specific phospholipase C releases immunoreactive Hyal2 into the aqueous phase. The protein is attached to the membrane through a functional GPI anchor. Hyal2 transfected in COS-7 cells is associated with detergent-resistant, cholesterol-rich membranes known as lipid rafts	Homo sapiens	16020	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
52000	-	x * 52000, SDS-PAGE	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q12891	-	-

Purification (Commentary)

Purification (Comment)	Organism
Hyal2 is strongly associated with cell membrane fractions from which it can be extracted using a Triton X-114 treatment, but not an osmotic shock or an alkaline carbonate solution. Treatment of membrane preparations with phosphatidylinositol-specific phospholipase C releases immunoreactive Hyal2 into the aqueous phase. The protein is attached to the membrane through a functional GPI anchor. Hyal2 transfected in COS-7 cells is associated with detergent-resistant, cholesterol-rich membranes known as lipid rafts	Homo sapiens

Purification (Comment)

Organism

Hyal2 is strongly associated with cell membrane fractions from which it can be extracted using a Triton X-114 treatment, but not an osmotic shock or an alkaline carbonate solution. Treatment of membrane preparations with phosphatidylinositol-specific phospholipase C releases immunoreactive Hyal2 into the aqueous phase. The protein is attached to the membrane through a functional GPI anchor. Hyal2 transfected in COS-7 cells is associated with detergent-resistant, cholesterol-rich membranes known as lipid rafts

Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
MDA-MB-231 cell	high expression	Homo sapiens	-

Subunits

Subunits	Comment	Organism
?	x * 52000, SDS-PAGE	Homo sapiens

Synonyms

Synonyms	Comment	Organism
Hyal2	-	Homo sapiens
LUCA2	-	Homo sapiens