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Literature summary for 3.2.1.35 extracted from

  • Kaessler, A.; Nourrisson, M.R.; Duflos, M.; Jose, J.
    Indole carboxamides inhibit bovine testes hyaluronidase at pH 7.0 and indole acetamides activate the enzyme at pH 3.5 by different mechanisms (2008), J. Enzyme Inhib. Med. Chem., 23, 719-727.
    View publication on PubMed
Search for more literature for 3.2.1.35

Activating Compound

Activating Compound Comment Organism Structure
bovine serum albumine
-
 Bos taurus
N-(4,6-dimethylpyridin-2-yl)-(1-ethylindole-3-yl)acetamide acts as an inhibitor at pH 7 and at pH 3.5 activity is increased to 134% Bos taurus
N-(4,6-dimethylpyridin-2-yl)-[5-bromo-1-(4-methyl)indole-3-yl]carboxamide acts as an inhibitor at pH 7 and at pH 3.5 activity is increased to 117% Bos taurus
N-(pyridin-4yl)-[5-bromo-1-(4-fluorobenzyl)indole-3-yl]carboxamide acts as an inhibitor at pH 7 and at pH 3.5 activity is increased to 120% Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
ascorbic acid palmitate inhibition at pH 7: 99%, at pH 3.5: 99% Bos taurus
N-(4,6-dimethylpyridin-2-yl)-(1-ethylindole-3-yl)acetamide inhibition at pH 7: 3%, but at pH 3.5: 134% activation Bos taurus
N-(4,6-dimethylpyridin-2-yl)-[5-bromo-1-(4-methyl)indole-3-yl]carboxamide inhibition at pH 7: 37%, but at pH 3.5: 117% activation Bos taurus
N-(pyridin-4yl)-[5-bromo-1-(4-fluorobenzyl)indole-3-yl]carboxamide inhibition at pH 7: 50%, but at pH 3.5: 120% activation Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
testis
-
 Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
hyaluronic acid + H2O
-
 Bos taurus ?
-
 ?

Synonyms

Synonyms Comment Organism
hyaluronidase
-
 Bos taurus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Bos taurus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.008
-
 pH: 7.0 Bos taurus ascorbic acid palmitate
0.018
-
 pH: 3.5 Bos taurus ascorbic acid palmitate
0.046
-
 pH: 7.0 Bos taurus N-(pyridin-4yl)-[5-bromo-1-(4-fluorobenzyl)indole-3-yl]carboxamide
0.078
-
 pH: 7.0 Bos taurus N-(4,6-dimethylpyridin-2-yl)-[5-bromo-1-(4-methyl)indole-3-yl]carboxamide