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Literature summary for 3.2.1.35 extracted from

  • Chen, F.; Kakizaki, I.; Yamaguchi, M.; Kojima, K.; Takagaki, K.; Endo, M.
    Novel products in hyaluronan digested by bovine testicular hyaluronidase (2008), Glycoconj. J., 26, 559-566.
    View publication on PubMed
Search for more literature for 3.2.1.35

Application

Application Comment Organism
biotechnology novel products in hyaluronan digested by commercially available bovine testicular hyaluronidase are found which originate from a novel enzyme contaminat in the BTH Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
testis
-
 Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chondroitin + H2O
-
 Bos taurus ?
-
 ?
chondroitin 4-sulfate + H2O
-
 Bos taurus ?
-
 ?
chondroitin 6-sulfate + H2O
-
 Bos taurus ?
-
 ?
hyaluronan + H2O HPLC and MS analyses of the products reveal the existance of oligosaccharides which are not produced under hydrolysis conditions, but during transglycosylation by the enzyme, suggesting that a novel enzyme is present as a contaminant in commercial BTH, which acts as an N-deacetylase of N-acetylglucosamine at the reducing terminal of hyaluronan oligosaccharides Bos taurus ?
-
 ?

Synonyms

Synonyms Comment Organism
BTH
-
 Bos taurus
hyaluronidase
-
 Bos taurus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 assay at Bos taurus