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Literature summary for 3.2.1.35 extracted from
- Purification and properties of hyaluronidase from Hippasa partita (funnel web spider) venom gland extract (2007), Toxicon, 50, 383-393.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| NaCl | optimal activity in the presence of 300 mM NaCl | Hippasa partita |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | inhibition at 300 mM | Hippasa partita | |
| Mg2+ | inhibition at 300 mM | Hippasa partita | |
| Zn2+ | inhibition at 300 mM | Hippasa partita | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | - |
Hippasa partita | |
| additional information | not activated by Cl- | Hippasa partita | |
| Na+ | - |
Hippasa partita | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42260 | - |
MALDI-TOF mass spectrometry | Hippasa partita |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Hippasa partita | - |
funnel web spider | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Sephadex G-100 column chromatography and CM-Sephadex C-25 column chromatography | Hippasa partita |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| venom | - |
Hippasa partita | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.00184 | - |
venom gland extract, at 37°C | Hippasa partita |
| 0.03767 | - |
after 20.4fold purification, at 37°C | Hippasa partita |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| hyaluronan + 2 H2O | absolute specificity for hyaluronan | Hippasa partita | N-acetyl-beta-D-glucosamine + D-glucuronate + ? | - |
? | |
| additional information | chondroitin, chondroitin sulfate A, chondroitin sulfate B, chondroitin sulfate C, chondroitin sulfate D, heparin, and chitosan are no substrates | Hippasa partita | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Hyal | - |
Hippasa partita |
| hyaluronidase | - |
Hippasa partita |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Hippasa partita |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.8 | - |
- |
Hippasa partita |
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