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Literature summary for 3.2.1.35 extracted from
- Cloning and molecular characterization of the first aquatic hyaluronidase, SFHYA1, from the venom of stonefish (Synanceja horrida) (2005), Gene, 346, 71-81.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| precursor from venom gland, DNA and amino acid sequence determination and analysis, phylogenetic analysis and sequence comparisons, functional expression of His-tagged enzyme in insect cells, but not in Escherichia coli, and secretion of the recombinant enzyme, overview | Synanceia horrida |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | venom | Synanceia horrida | - |
- |
| intracellular | venom gland enzyme | Synanceia horrida | 5622 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Synanceia horrida | Q801Z8 | stonefish | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | the enzyme contains three potential N-glycosylation sites | Synanceia horrida |
| proteolytic modification | the precursor enzyme contains a signal sequence for targeting into endoplasmic reticulum | Synanceia horrida |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme from lyophilized venom by affinity chromatography | Synanceia horrida |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| hyaluronic acid hexasaccharide + H2O = hyaluronic acid disaccharide + hyaluronic acid tetrasaccharide | the enzyme contains four hyaluronan binding sites [B(X)7B] | Synanceia horrida |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| venom | - |
Synanceia horrida | - |
| venom gland | precursor | Synanceia horrida | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| hyaluronan + H2O | human substrate | Synanceia horrida | hyaluronan oligomers | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| hyaluronidase | - |
Synanceia horrida |
| SFHYA1 | - |
Synanceia horrida |
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Release 2023.1 (January 2023)
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