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Literature summary for 3.2.1.35 extracted from

  • Stern, R.; Jedrzejas, M.J.
    Hyaluronidases: their genomics, structures, and mechanisms of action (2006), Chem. Rev., 106, 818-839.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
isozymes are clustered on chromosome 9F1-F2 and 6A2, expression profiles Mus musculus
isozymes Hyal1-Hyal4, sequence comparison, the genes encoding isozymes Hyal1-Hyal3 are tightly clustered on chromosome 3q21.3, Hyal4 and HPH-20 are clustered on 7q31.3, expression profiles Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
three-dimensional structure analysis Bos taurus
three-dimensional structure analysis Apis mellifera

Protein Variants

Protein Variants Comment Organism
D146N site-directed mutagenesis of HPH-20, the mutant shows highly reduced activity Homo sapiens
E148Q site-directed mutagenesis of HPH-20, inactive mutant Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
acrosome spermatid, HPH-20 Homo sapiens
-
-
extracellular venom Apis mellifera
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
hyaluronan + H2O Mus musculus
-
hyaluronan oligomers
-
?
hyaluronan + H2O Bos taurus
-
hyaluronan oligomers
-
?
hyaluronan + H2O Apis mellifera
-
hyaluronan oligomers
-
?
hyaluronan + H2O Homo sapiens the enzyme is required for fertility facilitating the penetration of sperm through the cumulus mass in the ovum hyaluronan oligomers
-
?
additional information Homo sapiens HPH-20 or SPAM1, sperm adhesion molecule 1, is a bifunctional enzyme, which is also an adhesion molecule with binding properties to the cumulus mass surrounding the ovum ?
-
?

Organism

Organism UniProt Comment Textmining
Apis mellifera Q08169
-
-
Bos taurus
-
-
-
Homo sapiens
-
isozymes Hyal1-Hyal4, and HPH-20, or SPAM1
-
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Bos taurus

Reaction

Reaction Comment Organism Reaction ID
hyaluronic acid hexasaccharide + H2O = hyaluronic acid disaccharide + hyaluronic acid tetrasaccharide reaction mechanism and three-dimensional structure Mus musculus
hyaluronic acid hexasaccharide + H2O = hyaluronic acid disaccharide + hyaluronic acid tetrasaccharide reaction mechanism and three-dimensional structure, catalytic residue is Glu113, substrate positioning residues, overview Apis mellifera
hyaluronic acid hexasaccharide + H2O = hyaluronic acid disaccharide + hyaluronic acid tetrasaccharide reaction mechanism and three-dimensional structure, catalytic residue is Glu149, substrate positioning residues, overview Bos taurus
hyaluronic acid hexasaccharide + H2O = hyaluronic acid disaccharide + hyaluronic acid tetrasaccharide reaction mechanism and three-dimensional structure, catalytic residues of isozymes Hyal1-Hyal4 are Glu131, Glu135, Glu129, and Glu147, respectively, substrate positioning residues, active site structure of isozymes, overview Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
breast HPH-20 Homo sapiens
-
commercial preparation testicular Bos taurus
-
epididymis HPH-20 Homo sapiens
-
ovary HPH-20 Homo sapiens
-
spermatid HPH-20 Homo sapiens
-
spermatozoon
-
Homo sapiens
-
testis
-
Bos taurus
-
testis HPH-20 Homo sapiens
-
uterus HPH-20 Homo sapiens
-
venom
-
Apis mellifera
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
commercial preparation Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
hyaluronan + H2O
-
Mus musculus hyaluronan oligomers
-
?
hyaluronan + H2O
-
Homo sapiens hyaluronan oligomers
-
?
hyaluronan + H2O
-
Bos taurus hyaluronan oligomers
-
?
hyaluronan + H2O
-
Apis mellifera hyaluronan oligomers
-
?
hyaluronan + H2O the enzyme is required for fertility facilitating the penetration of sperm through the cumulus mass in the ovum Homo sapiens hyaluronan oligomers
-
?
additional information HPH-20 or SPAM1, sperm adhesion molecule 1, is a bifunctional enzyme, which is also an adhesion molecule with binding properties to the cumulus mass surrounding the ovum Homo sapiens ?
-
?

Subunits

Subunits Comment Organism
More three-dimensional structure analysis Bos taurus
More three-dimensional structure analysis Apis mellifera
More three-dimensional structure analysis and modeling of isozymes, structure-reaction mechanism relationship, overview Homo sapiens

Synonyms

Synonyms Comment Organism
BPH-20
-
Bos taurus
HPH-20
-
Homo sapiens
human PH-20
-
Homo sapiens
Hyal
-
Homo sapiens
hyaluronidase
-
Mus musculus
hyaluronidase
-
Homo sapiens
hyaluronidase
-
Bos taurus
hyaluronidase
-
Apis mellifera
SPAM1
-
Homo sapiens
spreading factor
-
Homo sapiens