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Literature summary for 3.2.1.35 extracted from
- A low molecular weight isoform of hyaluronidase: purification from Indian cobra (Naja naja) venom and partial characterization (2005), Biochemistry (Moscow), 70, 708-712.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| curcumin | IC50 is 0.057 mM | Naja naja |  |
| indomethacin | IC50 is 0.086 mM | Naja naja | |
| Tannic acid | IC50 is 0.070 mM | Naja naja | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | venom | Naja naja | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 52000 | - |
1 * 52000, isozyme NNH2, SDS-PAGE | Naja naja |
| 54000 | - |
isozyme NNH2, gel filtration | Naja naja |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| hyaluronan + H2O | Naja naja | human skin sections | hyaluronan oligomers | - |
? | |
| additional information | Naja naja | isozyme NNH2 is nontoxic, but indirectly potentiates the hemorrhagic activity of hemorrhagic complex-I | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Naja naja | - |
isozyme NNH2 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native isozyme NNH2 26fold to homogeneity from venom by successive gel filtration and ion exchange chromatography | Naja naja |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| venom | - |
Naja naja | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
isozyme NNH2 | Naja naja |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| hyaluronan + H2O | human skin sections | Naja naja | hyaluronan oligomers | - |
? | |
| hyaluronan + H2O | isozyme NNH2 shows endoglycosidase activity, absolute specific for in vitro, substrate from human skin | Naja naja | hyaluronan oligomers | - |
? | |
| additional information | isozyme NNH2 is nontoxic, but indirectly potentiates the hemorrhagic activity of hemorrhagic complex-I | Naja naja | ? | - |
? | |
| additional information | no activity with chondroitin sulfates, heparin, and different chitosans | Naja naja | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 52000, isozyme NNH2, SDS-PAGE | Naja naja |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| hyaluronidase | - |
Naja naja |
| NNH2 | - |
Naja naja |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Naja naja | isoelectric focusing, isozyme NNH2 | - |
9.7 |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.057 | - |
IC50 is 0.057 mM | Naja naja | curcumin | |
| 0.07 | - |
IC50 is 0.070 mM | Naja naja | Tannic acid | |
| 0.086 | - |
IC50 is 0.086 mM | Naja naja | indomethacin | |
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Release 2023.1 (January 2023)
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