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Literature summary for 3.2.1.33 extracted from

  • Liu, W.; Madsen, N.B.; Fan, B.; Zucker, K.A.; Glew, R.H.; Fry, D.E.
    Effects of oligosaccharide binding on glycogen debranching enzyme activity and conformation (1995), Biochemistry, 34, 7056-7061.
    View publication on PubMed
Search for more literature for 3.2.1.33

Activating Compound

Activating Compound Comment Organism Structure
alpha-cyclodextrin 20 mM: 2.3fold activation Oryctolagus cuniculus
amylopectin potent activator Oryctolagus cuniculus
glucooligosaccharides with four or more glucose units, uncompetitive activation Oryctolagus cuniculus
glycogen potent activator Oryctolagus cuniculus
maltohexaose 20 mM: 1.86fold activation Oryctolagus cuniculus
maltopentaose 20 mM: 1.45fold activation Oryctolagus cuniculus
maltotetraose 20 mM: 1.15fold activation Oryctolagus cuniculus
additional information mechanism of activation Oryctolagus cuniculus

Inhibitors

Inhibitors Comment Organism Structure
D-glucose competitive Oryctolagus cuniculus
glucooligosaccharides containing one, two, or three glucose, competitive inhibitors Oryctolagus cuniculus
maltose competitive Oryctolagus cuniculus
maltotriose competitive Oryctolagus cuniculus
additional information inhibition mechanism Oryctolagus cuniculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetic data Oryctolagus cuniculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glycogen phosphorylase-limit dextrin + H2O Oryctolagus cuniculus
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
glycogen debranching enzyme Oryctolagus cuniculus

Reaction

Reaction Comment Organism Reaction ID
6-O-alpha-D-glucosyl cyclomaltoheptaose + H2O = D-glucose + cyclomaltoheptaose mechanism Oryctolagus cuniculus
a

Source Tissue

Source Tissue Comment Organism Textmining
muscle
-
 Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
11
-
-
 Oryctolagus cuniculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-D-glucosyl fluoride
-
 Oryctolagus cuniculus fluoride + D-glucose
-
 ?
glycogen + H2O slowly hydrolyzed Oryctolagus cuniculus glycogen + D-glucose
-
 r
glycogen phosphorylase-limit dextrin + H2O
-
 Oryctolagus cuniculus limit dextrin + D-glucose
-
 r
glycogen phosphorylase-limit dextrin + H2O
-
 Oryctolagus cuniculus ?
-
 ?

Synonyms

Synonyms Comment Organism
glycogen debranching system EC 3.2.1.33 found in mammals and yeast is in a single polypeptide chain containing two active centres. The other activity is similar to that of EC 2.4.1.25, 4-alpha-glucanotransferase, which acts on the glycogen phosphorylase limit dextrin chains to expose the single glucose residues, which the 6-alpha-glucosidase activity can hydrolyse. Together, these two activities constitute the glycogen debranching system Oryctolagus cuniculus