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Literature summary for 3.2.1.33 extracted from
- Amylo-1,6-glucosidase/4-alpha-glucanotransferase. Reaction of rabbit muscle debranching enzyme with an active site-directed irreversible inhibitor, 1-S-dimethylarsino-1-thio-beta-D-glucopyranoside (1980), J. Biol. Chem., 255, 8451-8457.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1-S-dimethylarsino-1-thio-beta-D-glucanopyranoside | DATG, active site-directed irreversible inhibitor, inhibition: mechanism. Inactivation occurs with significant conformational change | Mammalia |  |
| 1-S-dimethylarsino-1-thio-beta-D-glucanopyranoside | DATG, active site-directed irreversible inhibitor, inhibition: mechanism. Inactivation occurs with significant conformational change; glycogen phosphorylase limit dextrin and Bis-Tris protect from inactivation | Oryctolagus cuniculus | |
| 2,2-bis(hydroxymethyl)-2,2',2''-nitrilotriethanol | Bis-Tris, reversible inhibitor | Oryctolagus cuniculus | |
| cyclohexaamylose | alpha-Schardinger dextrin, debranching enzyme, competitive inhibitor | Oryctolagus cuniculus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetic mechanism | Oryctolagus cuniculus | |
| 43 | - |
D-glucose | - |
Oryctolagus cuniculus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glycogen phosphorylase-limit dextrin + H2O | Mammalia | phi-dextrin | ? | - |
? | |
| glycogen phosphorylase-limit dextrin + H2O | Oryctolagus cuniculus | phi-dextrin | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mammalia | - |
- |
- |
| Oryctolagus cuniculus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| glycogen debranching enzyme | Oryctolagus cuniculus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| 6-O-alpha-D-glucosyl cyclomaltoheptaose + H2O = D-glucose + cyclomaltoheptaose | mechanism | Oryctolagus cuniculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| muscle | - |
Mammalia | - |
| muscle | - |
Oryctolagus cuniculus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 6.5 | 8.9 | - |
Oryctolagus cuniculus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 50 mM Tris, 5 mM EDTA, 1 mM dithiothreitol, pH 7.2 | Oryctolagus cuniculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 63-alpha-glucosyl maltotetraose + H2O | branched pentasaccharide "fast B5" | Mammalia | maltotetraose + D-glucose | - |
r | |
| 63-alpha-glucosyl maltotetraose + H2O | branched pentasaccharide "fast B5" | Oryctolagus cuniculus | maltotetraose + D-glucose | - |
r | |
| alpha-(1-6)-glucosyl cyclohexaamylose + H2O | 6-alpha-glucosyl alpha-Schardinger dextrin, cyclodextrin | Mammalia | cyclohexaamylose + D-glucose | alpha-Schardinger dextrin | r | |
| alpha-(1-6)-glucosyl cyclohexaamylose + H2O | 6-alpha-glucosyl alpha-Schardinger dextrin, cyclodextrin | Oryctolagus cuniculus | cyclohexaamylose + D-glucose | alpha-Schardinger dextrin | r | |
| glycogen + H2O | - |
Mammalia | glycogen + D-glucose | - |
r | |
| glycogen + H2O | reverse reaction: synthesis of (1, 6)-bound side chains, reincorporation of glucose into polysaccharide | Oryctolagus cuniculus | glycogen + D-glucose | - |
r | |
| glycogen phosphorylase-limit dextrin + H2O | phi-dextrin | Mammalia | limit dextrin + D-glucose | - |
r | |
| glycogen phosphorylase-limit dextrin + H2O | phi-dextrin | Oryctolagus cuniculus | limit dextrin + D-glucose | debranched phi-dextrin | r | |
| glycogen phosphorylase-limit dextrin + H2O | phi-dextrin | Mammalia | ? | - |
? | |
| glycogen phosphorylase-limit dextrin + H2O | phi-dextrin | Oryctolagus cuniculus | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| glycogen debranching system | EC 3.2.1.33 found in mammals and yeast is in a single polypeptide chain containing two active centres. The other activity is similar to that of EC 2.4.1.25, 4-alpha-glucanotransferase, which acts on the glycogen phosphorylase limit dextrin chains to expose the single glucose residues, which the 6-alpha-glucosidase activity can hydrolyse. Together, these two activities constitute the glycogen debranching system | Mammalia |
| glycogen debranching system | EC 3.2.1.33 found in mammals and yeast is in a single polypeptide chain containing two active centres. The other activity is similar to that of EC 2.4.1.25, 4-alpha-glucanotransferase, which acts on the glycogen phosphorylase limit dextrin chains to expose the single glucose residues, which the 6-alpha-glucosidase activity can hydrolyse. Together, these two activities constitute the glycogen debranching system | Oryctolagus cuniculus |
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