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Literature summary for 3.2.1.32 extracted from

  • Goddard-Borger, E.D.; Sakaguchi, K.; Reitinger, S.; Watanabe, N.; Ito, M.; Withers, S.G.
    Mechanistic insights into the 1,3-xylanases: useful enzymes for manipulation of algal biomass (2012), J. Am. Chem. Soc., 134, 3895-3902.
    View publication on PubMed
Search for more literature for 3.2.1.32

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Vibrio sp.

Inhibitors

Inhibitors Comment Organism Structure
(3R,4R)-4-hydroxy-3-[beta-D-xylopyranosyl-(1->3)-beta-D-xylopyranosyloxy]-piperidine competitive Vibrio sp.
(3R,4R)-4-hydroxy-3-[beta-D-xylopyranosyloxy]-piperidine competitive Vibrio sp.
22,4-dinitrophenyl-beta-D-xylopyranosyl-(1->3)-beta-D-xylopyranosyl-(1->3)2-deoxy-2-fluoro-beta-D-xylopyranoside
-
 Vibrio sp.
phenyl 1-thio-beta-D-xylopyranoside
-
 Vibrio sp.
phenyl 4-O-beta-D-xylopyranosyl-1-thio-beta-D-xylopyranoside
-
 Vibrio sp.
phenyl beta-D-xylopyranosyl-(1->3)-1-thio-beta-D-xylopyranoside
-
 Vibrio sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0038
-
 2-oxo-4-(trifluoromethyl)-2H-chromen-7-yl beta-D-xylopyranosyl-(1->3)-beta-D-xylopyranosyl-(1->3)-beta-D-xylopyranoside at pH 7.5 and 37°C Vibrio sp.
1.7
-
 2-oxo-4-(trifluoromethyl)-2H-chromen-7-yl 3-O-beta-D-xylopyranosyl-beta-D-xylopyranoside at pH 7.5 and 37°C Vibrio sp.
71
-
 2-oxo-4-(trifluoromethyl)-2H-chromen-7-yl 3-O-(3-O-methyl-etab-D-xylopyranosyl)-beta-D-xylopyranoside at pH 7.5 and 37°C Vibrio sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
beta-1,3-xylan + H2O Vibrio sp.
-
 xylose + xylooligosaccharides
-
 ?

Organism

Organism UniProt Comment Textmining
Vibrio sp. D5MP61
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxo-4-(trifluoromethyl)-2H-chromen-7-yl 3-O-(3-O-methyl-etab-D-xylopyranosyl)-beta-D-xylopyranoside + H2O
-
 Vibrio sp. ?
-
 ?
2-oxo-4-(trifluoromethyl)-2H-chromen-7-yl 3-O-beta-D-xylopyranosyl-beta-D-xylopyranoside + H2O
-
 Vibrio sp. ?
-
 ?
2-oxo-4-(trifluoromethyl)-2H-chromen-7-yl beta-D-xylopyranosyl-(1->3)-beta-D-xylopyranosyl-(1->3)-beta-D-xylopyranoside + H2O
-
 Vibrio sp. beta-D-xylopyranosyl-(1->3)-beta-D-xylopyranosyl-(1->3)-beta-D-xylopyranose + 4-(trifluoromehtyl)umbelliferone
-
 ?
beta-1,3-xylan + H2O
-
 Vibrio sp. xylose + xylooligosaccharides
-
 ?
beta-1,3-xylan + H2O
-
 Vibrio sp. D-xylose + xylooligosaccharides
-
 ?
additional information no activity on 4-methylumbelliferyl beta-D-xylopyranosyl-(1->4)-beta-D-xyloside Vibrio sp. ?
-
 ?

Synonyms

Synonyms Comment Organism
endo-1,3-xylanase
-
 Vibrio sp.
XYL4
-
 Vibrio sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.89
-
 2-oxo-4-(trifluoromethyl)-2H-chromen-7-yl beta-D-xylopyranosyl-(1->3)-beta-D-xylopyranosyl-(1->3)-beta-D-xylopyranoside at pH 7.5 and 37°C Vibrio sp.
1.9
-
 2-oxo-4-(trifluoromethyl)-2H-chromen-7-yl 3-O-beta-D-xylopyranosyl-beta-D-xylopyranoside at pH 7.5 and 37°C Vibrio sp.
2.1
-
 2-oxo-4-(trifluoromethyl)-2H-chromen-7-yl 3-O-(3-O-methyl-etab-D-xylopyranosyl)-beta-D-xylopyranoside at pH 7.5 and 37°C Vibrio sp.

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.002
-
 (3R,4R)-4-hydroxy-3-[beta-D-xylopyranosyl-(1->3)-beta-D-xylopyranosyloxy]-piperidine at pH 7.5 and 37°C Vibrio sp.
0.0031
-
 (3R,4R)-4-hydroxy-3-[beta-D-xylopyranosyloxy]-piperidine at pH 7.5 and 37°C Vibrio sp.

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1
-
 2-oxo-4-(trifluoromethyl)-2H-chromen-7-yl 3-O-beta-D-xylopyranosyl-beta-D-xylopyranoside at pH 7.5 and 37°C Vibrio sp.
3
-
 2-oxo-4-(trifluoromethyl)-2H-chromen-7-yl 3-O-(3-O-methyl-etab-D-xylopyranosyl)-beta-D-xylopyranoside at pH 7.5 and 37°C Vibrio sp.
230
-
 2-oxo-4-(trifluoromethyl)-2H-chromen-7-yl beta-D-xylopyranosyl-(1->3)-beta-D-xylopyranosyl-(1->3)-beta-D-xylopyranoside at pH 7.5 and 37°C Vibrio sp.