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Literature summary for 3.2.1.32 extracted from

  • Aoki, T.; Araki, T.; Kitamikado, M.
    Purification and characterization of an endo-beta-1,3-xylanase from Vibrio sp. (1988), Nippon Suisan Gakkaishi, 54, 277-281.
No PubMed abstract available
Search for more literature for 3.2.1.32

Inhibitors

Inhibitors Comment Organism Structure
Ag+
-
 Vibrio sp.
Cu2+
-
 Vibrio sp.
Fe3+ 1 mM, complete inhibition Vibrio sp.
Hg2+ 1 mM, complete inhibition Vibrio sp.
Mn2+
-
 Vibrio sp.
Pb2+
-
 Vibrio sp.
PCMB
-
 Vibrio sp.
Zn2+
-
 Vibrio sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Vibrio sp.
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
 gel filtration Vibrio sp.

Organism

Organism UniProt Comment Textmining
Vibrio sp.
-
 AX-4
-

Purification (Commentary)

Purification (Comment) Organism
-
 Vibrio sp.

Source Tissue

Source Tissue Comment Organism Textmining
culture medium
-
 Vibrio sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
13.6
-
-
 Vibrio sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-1,3-xylan + H2O
-
 Vibrio sp. xylose + xylooligosaccharides
-
 ?
additional information does not act on xylobiose Vibrio sp. ?
-
 ?
xylopentaose + H2O
-
 Vibrio sp. xylose + xylotetraose + xylobiose + xylotriose small amounts of xylobiose and xylotriose ?
xylotetraose + H2O
-
 Vibrio sp. xylose + xylotriose + xylobiose small amount of xylobiose ?
xylotriose + H2O
-
 Vibrio sp. xylose + xylobiose
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 pH 6.0, 15 min, stable below Vibrio sp.
55
-
 pH 6.0, 15 min, complete loss of activity Vibrio sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 6.5
-
 Vibrio sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.5 10 4°C, 20 h, stable Vibrio sp.