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Literature summary for 3.2.1.31 extracted from

  • Beaud, D.; Tailliez, P.; Anba-Mondoloni, J.
    Genetic characterization of the beta-glucuronidase enzyme from a human intestinal bacterium, Ruminococcus gnavus (2005), Microbiology, 151, 2323-2330.
    View publication on PubMed
Search for more literature for 3.2.1.31

Cloned(Commentary)

Cloned (Comment) Organism
gene uidA, DNA and amino acid sequence determination and analysis, genetic organization, expression analysis, expression in Escherichia coli strain TG1 mutant with inactivated endogenous enzyme activity, complementation of enzyme-deficient Escherichia coli strain [Ruminococcus] gnavus

Organism

Organism UniProt Comment Textmining
[Ruminococcus] gnavus Q6W7J7 isolated from human intestinal microflora, gene uidA
-
[Ruminococcus] gnavus E1 Q6W7J7 isolated from human intestinal microflora, gene uidA
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information activity during cell growth [Ruminococcus] gnavus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.08
-
 activity in crude cell extract from cell after growth for 9 h, activity-growth curve [Ruminococcus] gnavus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-glucuronide + H2O
-
 [Ruminococcus] gnavus 4-nitrophenol + beta-D-glucuronic acid
-
 ?
4-nitrophenyl beta-D-glucuronide + H2O
-
 [Ruminococcus] gnavus E1 4-nitrophenol + beta-D-glucuronic acid
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at [Ruminococcus] gnavus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
 assay at [Ruminococcus] gnavus