Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-chloromercuriphenylsulfonic acid | inhibition of isozymes I and II | Streptococcus sp. | |
Cu2+ | inhibition of isozymes I and II | Streptococcus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
67000 | - |
2 * 67000, isozymes I and II, SDS-PAGE | Streptococcus sp. |
140000 | - |
isozymes I and II, gel filtration | Streptococcus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus sp. | - |
isolated from human intestinal microflora, two isozymes I and II | - |
Streptococcus sp. LJ-22 | - |
isolated from human intestinal microflora, two isozymes I and II | - |
Purification (Comment) | Organism |
---|---|
native isozymes I and II to homogeneity by ammonium sulfate fractionation, hydrophobic interaction, ion exchange, and hydroxyapatite chromatographies, and glycyrrhizin affinity chromatography | Streptococcus sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.137 | - |
purified isozyme I | Streptococcus sp. |
0.19 | - |
purified isozyme II | Streptococcus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycyrrhizin + H2O | specific substrate of isozymes I and II | Streptococcus sp. | 18beta-glycyrrhetinic acid-3-O-beta-D-glucuronide + beta-D-glucuronic acid | - |
? | |
glycyrrhizin + H2O | specific substrate of isozymes I and II | Streptococcus sp. LJ-22 | 18beta-glycyrrhetinic acid-3-O-beta-D-glucuronide + beta-D-glucuronic acid | - |
? | |
additional information | no activity of isozymes I and II with 4-nitrophenyl beta-D-glucuronide, baicalin, and 18beta-glycyrrhetinic acid-3-O-beta-D-glucuronide | Streptococcus sp. | ? | - |
? | |
additional information | no activity of isozymes I and II with 4-nitrophenyl beta-D-glucuronide, baicalin, and 18beta-glycyrrhetinic acid-3-O-beta-D-glucuronide | Streptococcus sp. LJ-22 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 67000, isozymes I and II, SDS-PAGE | Streptococcus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
purified isozyme II | Streptococcus sp. |
7 | - |
purified isozyme I | Streptococcus sp. |