Literature summary for 3.2.1.31 extracted from

Nakamura, T.; Takagaki, K.; Majima, M.; Kimura, S.; Kubo, K.; Endo, M.
A new type of exo-beta-glucuronidase acting only on non-sulfated glycosaminoglycans (1990), J. Biol. Chem., 265, 5390-5397.

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General Stability

General Stability	Organism
the enzyme acting on chondroitin is less stable than enzyme acting on p-nitrophenylphosphate	Oryctolagus cuniculus

Inhibitors

Inhibitors	Comment	Organism	Structure
NaCl	between 0.075 and 0.25 M, the activity is decreased with increasing NaCl concentration. Above 0.25 M, the enzyme shows no activity. The activity of beta-glucuronidase acting on p-nitrophenyl-beta-D-glucuronide is decreased with increasing NaCl concentrations, and 0.4 M NaCl produces 50% inhibition	Oryctolagus cuniculus
saccharo-1,4-lactone	-	Oryctolagus cuniculus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
6.3	-	chondroitin	pH 5.0, 37°C	Oryctolagus cuniculus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
NaCl	below 0.075 M the activity of beta-glucuronidase acting on chondroitin is increased with increasing NaCl concentrations.The activity of beta-glucuronidase acting on p-nitrophenyl-beta-D-glucuronide is decreased with increasing NaCl concentrations, and 0.4 M NaCl produces 50% inhibition	Oryctolagus cuniculus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
75000	-	x * 75000, SDS-PAGE, beta-glucuronidase acting on p-nitrophenyl-beta-D-glucuronide	Oryctolagus cuniculus
130000	-	gel filtration, beta-glucuronidase acting on chondroitin	Oryctolagus cuniculus
280000	300000	gel filtration, beta-glucuronidase acting on p-nitrophenyl-beta-D-glucuronide	Oryctolagus cuniculus

Organism

Organism	UniProt	Comment	Textmining
Oryctolagus cuniculus	-	2 different exo-beta-glucuronidases: a beta-glucuronidase acting on p-nitrophenyl-beta-D-glucuronide and a beta-glucuronidase acting on chondroitin	-

Purification (Commentary)

Purification (Comment)	Organism
2 different exo-beta-glucuronidases: a beta-glucuronidase acting on p-nitrophenyl-beta-D-glucuronide and a beta-glucuronidase acting on chondroitin	Oryctolagus cuniculus

Source Tissue

Source Tissue	Comment	Organism	Textmining
liver	-	Oryctolagus cuniculus	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.127	-	beta-glucuronidase acting on chondroitin	Oryctolagus cuniculus

Storage Stability

Storage Stability	Organism
-20°C, enzyme acting on chondroitin retains high level of activity for at least 1 month	Oryctolagus cuniculus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(4GlcAbeta1-3GalNAc(4-OSO3-)beta1-)2 + H2O	no activity with enzyme acting on chondroitin, 142% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on p-nitrophenyl-beta-D-glucuronide	Oryctolagus cuniculus	D-glucuronate + ?	-	?
(4GlcAbeta1-3GalNAc(4-OSO3-)beta1-)3 + H2O	no activity with enzyme acting on chondroitin, 63% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on p-nitrophenyl-beta-D-glucuronide	Oryctolagus cuniculus	D-glucuronate + ?	-	?
(4GlcAbeta1-3GalNAc(6-OSO3-)beta1-)2 + H2O	no activity with enzyme acting on chondroitin, 50% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on p-nitrophenyl-beta-D-glucuronide	Oryctolagus cuniculus	D-glucuronate + ?	-	?
(4GlcAbeta1-3GalNAc(6-OSO3-)beta1-)3 + H2O	no activity with enzyme acting on chondroitin, 33% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on p-nitrophenyl-beta-D-glucuronide	Oryctolagus cuniculus	D-glucuronate + ?	-	?
(4GlcAbeta1-3GalNAcbeta1-)2 + H2O	66% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on chondroitin, 90% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on p-nitrophenyl-beta-D-glucuronide	Oryctolagus cuniculus	D-glucuronate + ?	-	?
(4GlcAbeta1-3GalNAcbeta1-)3 + H2O	56% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on chondroitin, 71% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on p-nitrophenyl-beta-D-glucuronide	Oryctolagus cuniculus	D-glucuronate + ?	-	?
(4GlcAbeta1-3GlcNAcbeta1-)3 + H2O	81% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on chondroitin, 73% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on p-nitrophenyl-beta-D-glucuronide	Oryctolagus cuniculus	D-glucuronate + ?	-	?
chondroitin + H2O	MW 3500 Da: 23% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on chondroitin, 41% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on p-nitrophenyl-beta-D-glucuronide. MW 8000 Da: 11% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on chondroitin, 28% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on p-nitrophenyl-beta-D-glucuronide. MW 15000 Da: 6% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on chondroitin, 9% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on p-nitrophenyl-beta-D-glucuronide	Oryctolagus cuniculus	D-glucuronate + ?	-	?
GlcAbeta1-3GalNAc(4-OSO3-) + H2O	no activity with enzyme acting on chondroitin, 4% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on p-nitrophenyl-beta-D-glucuronide	Oryctolagus cuniculus	D-glucuronate + N-acetylamino-beta-D-galactopyranose 4-sulfate	-	?
additional information	no activity with enzyme acting on chondroitin: N-acetylhyalobiuronic acid, N-acetylchondrosine, (4GlcAbeta1-3GalNAc(4-OSO3-)beta1-)3, (4GlcAbeta1-3GalNAc(4-OSO3-)beta1-)2, GlcAbeta1-3galNAc(4-OSO3-), (4GlcAbeta1-3GalNAc(6-OSO3-)beta1-)3, (4GlcAbeta1-3GalNAc(6-OSO3-)beta1-)2. No activity with enzyme acting on p-nitrophenyl-beta-D-glucuronide: N-acetylhyalobiuronic acid, N-acetylchondrosine	Oryctolagus cuniculus	?	-	?
p-nitrophenyl-beta-D-glucuronide + H2O	1% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on chondroitin, 192% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on p-nitrophenyl-beta-D-glucuronide	Oryctolagus cuniculus	D-glucuronate + ?	-	?

Subunits

Subunits	Comment	Organism
?	x * 75000, SDS-PAGE, beta-glucuronidase acting on p-nitrophenyl-beta-D-glucuronide	Oryctolagus cuniculus
More	beta-glucuronidase acting on chondroitin yields several protein bands on SDS-PAGE, no protein band with a MW of 75000 Da	Oryctolagus cuniculus

Synonyms

Synonyms	Comment	Organism
exo-beta-glucuronidase	-	Oryctolagus cuniculus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
4.5	-	beta-glucuronidase acting on p-nitrophenyl-beta-D-glucuronide	Oryctolagus cuniculus
5	-	beta-glucuronidase acting on chondroitin	Oryctolagus cuniculus

pH Range

pH Minimum	pH Maximum	Comment	Organism
3	5	pH 3.0: about 50% of maximal activity, pH 5.0: about 70% of maximal activity, beta-glucuronidase acting on p-nitrophenyl-beta-D-glucuronide	Oryctolagus cuniculus
4	5.5	pH 4.0: about 45% of maximal activity, pH 5.5: about 90% of maximal activity, pH 6.0: no activity, beta-glucuronidase acting on chondroitin	Oryctolagus cuniculus

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Release 2023.1 (January 2023)