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Literature summary for 3.2.1.31 extracted from
- Purification and characterization of beta-glucuronidase from Escherichia coli HGU-3, a human intestinal bacterium (1995), Biol. Pharm. Bull., 18, 1184-1188.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | when beta-glucuronidase producing bacteria infect the bile, the pH of the bile becomes raised, the high pH and bile induce the enzyme, and then bilirubin gallstones can be easily formed | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CaCl2 | 2 mM, 14.8% inhibition | Escherichia coli |  |
| D-glucuronate | IC50: 7.5 mM, competitive | Escherichia coli | |
| FeCl2 | 2 mM,33.1% inhibition | Escherichia coli | |
| glycyrrhizin | IC50: 0.08 mM, competitive | Escherichia coli | |
| MgCl2 | 2 mM, 24.8% inhibition | Escherichia coli | |
| MnCl2 | 2 mM, 31.9% inhibition | Escherichia coli | |
| NEM | IC50: 0.09 mM | Escherichia coli | |
| NiCl2 | 2 mM, 52% inhibition | Escherichia coli | |
| p-chloromercuriphenylsulfonic acid | IC50: 0.04 mM | Escherichia coli | |
| p-nitrophenol | IC50: 0.3 mM, competitive | Escherichia coli | |
| saccharic acid 1,4-lactone | IC50: 0.09 mM, competitive | Escherichia coli | |
| silybin | IC50: 1 mM, non-competitive | Escherichia coli | |
| TLCK | IC50: 0.3 mM | Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.22 | - |
p-nitrophenyl-beta-D-glucuronide | pH 7.0, 37°C | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 72000 | - |
4 * 72000, SDS-PAGE | Escherichia coli |
| 290000 | - |
gel filtration | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Escherichia coli | when beta-glucuronidase producing bacteria infect the bile, the pH of the bile becomes raised, the high pH and bile induce the enzyme, and then bilirubin gallstones can be easily formed | ? | - |
? | |
| additional information | Escherichia coli HGU-3 | when beta-glucuronidase producing bacteria infect the bile, the pH of the bile becomes raised, the high pH and bile induce the enzyme, and then bilirubin gallstones can be easily formed | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
HGU-3 | - |
| Escherichia coli HGU-3 | - |
HGU-3 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 17.78 | - |
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| bilirubin diglucuronide + H2O | - |
Escherichia coli | bilirubin + D-glucuronate | - |
? | |
| bilirubin diglucuronide + H2O | - |
Escherichia coli HGU-3 | bilirubin + D-glucuronate | - |
? | |
| additional information | when beta-glucuronidase producing bacteria infect the bile, the pH of the bile becomes raised, the high pH and bile induce the enzyme, and then bilirubin gallstones can be easily formed | Escherichia coli | ? | - |
? | |
| additional information | when beta-glucuronidase producing bacteria infect the bile, the pH of the bile becomes raised, the high pH and bile induce the enzyme, and then bilirubin gallstones can be easily formed | Escherichia coli HGU-3 | ? | - |
? | |
| p-nitrophenyl-beta-D-glucuronide + H2O | - |
Escherichia coli | p-nitrophenol + D-glucuronate | - |
? | |
| p-nitrophenyl-beta-D-glucuronide + H2O | - |
Escherichia coli HGU-3 | p-nitrophenol + D-glucuronate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 72000, SDS-PAGE | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Escherichia coli |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8.5 | pH 5.0: about 45% of maximal activity, pH 8.5: about 50% of maximal activity | Escherichia coli |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Escherichia coli | chromatofocusing | - |
4.9 |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.04 | - |
IC50: 0.04 mM | Escherichia coli | p-chloromercuriphenylsulfonic acid | |
| 0.08 | - |
IC50: 0.08 mM, competitive | Escherichia coli | glycyrrhizin | |
| 0.09 | - |
IC50: 0.09 mM | Escherichia coli | NEM | |
| 0.09 | - |
IC50: 0.09 mM, competitive | Escherichia coli | saccharic acid 1,4-lactone | |
| 0.3 | - |
IC50: 0.3 mM | Escherichia coli | TLCK | |
| 0.3 | - |
IC50: 0.3 mM, competitive | Escherichia coli | p-nitrophenol | |
| 1 | - |
IC50: 1 mM, non-competitive | Escherichia coli | silybin | |
| 7.5 | - |
IC50: 7.5 mM, competitive | Escherichia coli | D-glucuronate | |
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