Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant expression of C-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha | Corallococcus coralloides |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | 18% activation at 1 mM, 25% inhibition at 5 mM | Corallococcus coralloides | |
Co2+ | complete inhibition at 1-5 mM | Corallococcus coralloides | |
Cr3+ | 62% inhibition at 1 mM, complete inhibition at 5 mM | Corallococcus coralloides | |
Cu2+ | 22% inhibition at 1 mM, complete inhibition at 5 mM | Corallococcus coralloides | |
EDTA | 22% inhibition at 10 mM | Corallococcus coralloides | |
Fe2+ | 27% inhibition at 1 mM, complete inhibition at 5 mM | Corallococcus coralloides | |
Fe3+ | 62% inhibition at 1 mM, complete inhibition at 5 mM | Corallococcus coralloides | |
Mn2+ | 34.5% inhibition at 1-5 mM | Corallococcus coralloides | |
Ni2+ | 44% inhibition at 1 mM, 70% at 5 mM | Corallococcus coralloides | |
SDS | 30% inhibition at 5 mg/ml | Corallococcus coralloides | |
Triton X-100 | 20% inhibition at 20 mg/ml | Corallococcus coralloides | |
Zn2+ | 28% inhibition at 1 mM, 43% at 5 mM | Corallococcus coralloides |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Corallococcus coralloides |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | 18% activation at 1 mM, 25% inhibition at 5 mM | Corallococcus coralloides | |
Ca2+ | 8.5% activation at 1 mM, 5.5% at 5 mM | Corallococcus coralloides | |
K+ | 34% activation at 1 mM, 31% at 5 mM | Corallococcus coralloides | |
Mg2+ | 50% activation at 1 mM, 8% at 5 mM | Corallococcus coralloides |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
starch + H2O | Corallococcus coralloides | - |
? | - |
? |
Organic Solvent | Comment | Organism |
---|---|---|
additional information | low concentrations (10%) of methanol, ethanol, acetonitrile, and DMSO do not affect the enzyme's activity | Corallococcus coralloides |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Corallococcus coralloides | A0A0H3WKG6 | - |
- |
Corallococcus coralloides CCTCC M2012528 | A0A0H3WKG6 | - |
- |
Corallococcus coralloides EGB | A0A0H3WKG6 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme 50.2fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Corallococcus coralloides |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
180 | - |
purified recombinant enzyme, pH 7.0, 50°C | Corallococcus coralloides |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-cyclodextrin + H2O | - |
Corallococcus coralloides | ? | - |
? | |
amylopectin + H2O | from potato | Corallococcus coralloides | ? | - |
? | |
amylose + H2O | from potato | Corallococcus coralloides | ? | - |
? | |
amylose + H2O | from potato | Corallococcus coralloides CCTCC M2012528 | ? | - |
? | |
amylose + H2O | from potato | Corallococcus coralloides EGB | ? | - |
? | |
dextran + H2O | - |
Corallococcus coralloides | ? | - |
? | |
dextran + H2O | - |
Corallococcus coralloides CCTCC M2012528 | ? | - |
? | |
dextran + H2O | - |
Corallococcus coralloides EGB | ? | - |
? | |
dextrin + H2O | - |
Corallococcus coralloides | ? | - |
? | |
dextrin + H2O | - |
Corallococcus coralloides CCTCC M2012528 | ? | - |
? | |
dextrin + H2O | - |
Corallococcus coralloides EGB | ? | - |
? | |
glycogen + H2O | - |
Corallococcus coralloides | ? | - |
? | |
additional information | substrate specificity, overview | Corallococcus coralloides | ? | - |
? | |
additional information | substrate specificity, overview | Corallococcus coralloides CCTCC M2012528 | ? | - |
? | |
additional information | substrate specificity, overview | Corallococcus coralloides EGB | ? | - |
? | |
pullulan + H2O | - |
Corallococcus coralloides | ? | - |
? | |
pullulan + H2O | - |
Corallococcus coralloides CCTCC M2012528 | ? | - |
? | |
pullulan + H2O | - |
Corallococcus coralloides EGB | ? | - |
? | |
starch + H2O | - |
Corallococcus coralloides | ? | - |
? | |
starch + H2O | soluble starch and raw starch from sweet potato and corn | Corallococcus coralloides | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 72920, about, sequence calculation | Corallococcus coralloides |
Synonyms | Comment | Organism |
---|---|---|
GHF15 glucoamylase | - |
Corallococcus coralloides |
GlucaM | - |
Corallococcus coralloides |
glucoamylase | - |
Corallococcus coralloides |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Corallococcus coralloides |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 80 | recombinant enzyme, activity range, profile overview | Corallococcus coralloides |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 80 | recombinant enzyme GlucaM retains full activity after 1 h of incubation at below 60°C and retains about 60% of the original activity after 1 h at 70°C, but it loses 60% of the original activity after 1 h of incubation at 80°C | Corallococcus coralloides |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Corallococcus coralloides |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 10 | recombinant enzyme, activity range, profile overview | Corallococcus coralloides |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Corallococcus coralloides | sequence calculation | - |
6.31 |