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Literature summary for 3.2.1.3 extracted from
- Enzymatic characterization of an extracellular glucoamylase from Tricholoma matsutake and its cloning and secretory expression in Pichia pastoris (2018), Biosci. Biotechnol. Biochem., 82, 2180-2190 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene TmGlu1, DNA and amino acid sequeence determination and analysis, recombinant secretory expression of the enzyme in Pichia pastoris strain X-33 | Tricholoma matsutake |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | complete inhibition | Tricholoma matsutake |  |
| Cu2+ | 14% inhibition at 5 mM | Tricholoma matsutake | |
| Fe2+ | 48% inhibition at 5 mM | Tricholoma matsutake | |
| Hg2+ | 52% inhibition at 5 mM | Tricholoma matsutake | |
| Pb2+ | 30% inhibition at 5 mM | Tricholoma matsutake | |
| Zn2+ | 27% inhibition at 5 mM | Tricholoma matsutake | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Tricholoma matsutake | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 29% activation at 5 mM | Tricholoma matsutake | |
| DTT | 25% activation at 5 mM | Tricholoma matsutake | |
| Mn2+ | slight activation at 5 mM | Tricholoma matsutake | |
| additional information | Ba2+, K+, Al3+, Mg2+, Fe3+, Na+, Ni+, Li+, Co2+, and EDTA have poor effects on activity | Tricholoma matsutake |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 63900 | - |
gel filtration | Tricholoma matsutake |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| starch + H2O | Tricholoma matsutake | soluble starch | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Tricholoma matsutake | H1AAU7 | - |
- |
| Tricholoma matsutake NBRC 30605 | H1AAU7 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Tricholoma matsutake |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native extracellular enzyme 33.2fold by ammonium sulfate fractionation, anion exchange chromatography, hydrophobic interaction chromatography, ultrafiltration, and gel filtration | Tricholoma matsutake |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| mycelium | high glucoamylase activity is induced upon culture of the fungus in a medium containing amylose compared with other saccharides such as glucose, rice starch, wheat starch, corn starch, and potato starch | Tricholoma matsutake | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 13.2 | - |
purified enzyme, pH 5.0, 60°C, with soluble starch as substrate | Tricholoma matsutake |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylopectin + H2O | - |
Tricholoma matsutake | ? | - |
? | |
| amylose A + H2O | - |
Tricholoma matsutake | ? | - |
? | |
| amylose B + H2O | - |
Tricholoma matsutake | ? | - |
? | |
| glycogen + H2O | - |
Tricholoma matsutake | ? | - |
? | |
| isomaltose + H2O | low activity | Tricholoma matsutake | ? | - |
? | |
| maltopentaose + H2O | - |
Tricholoma matsutake | ? | - |
? | |
| maltopentaose + H2O | - |
Tricholoma matsutake NBRC 30605 | ? | - |
? | |
| maltose + H2O | low activity | Tricholoma matsutake | ? | - |
? | |
| maltotetraose + H2O | - |
Tricholoma matsutake | ? | - |
? | |
| maltotetraose + H2O | - |
Tricholoma matsutake NBRC 30605 | ? | - |
? | |
| maltotriose + H2O | - |
Tricholoma matsutake | ? | - |
? | |
| maltotriose + H2O | - |
Tricholoma matsutake NBRC 30605 | ? | - |
? | |
| additional information | the enzyme degrades alpha-1,4- and alpha-1,6-glycosidic linkages in various polysaccharides and also malto-oligosaccharides, substrate specificity, overview. No activity with beta-cyclodextrin, alpha-cyclodextrin, trehalose, kojibiose, and nigerose | Tricholoma matsutake | ? | - |
? | |
| additional information | the enzyme degrades alpha-1,4- and alpha-1,6-glycosidic linkages in various polysaccharides and also malto-oligosaccharides, substrate specificity, overview. No activity with beta-cyclodextrin, alpha-cyclodextrin, trehalose, kojibiose, and nigerose | Tricholoma matsutake NBRC 30605 | ? | - |
? | |
| pullulan + H2O | - |
Tricholoma matsutake | ? | - |
? | |
| pullulan + H2O | - |
Tricholoma matsutake NBRC 30605 | ? | - |
? | |
| starch + H2O | soluble starch | Tricholoma matsutake | ? | - |
? | |
| starch + H2O | soluble starch, and low activity with raw corn starch | Tricholoma matsutake | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 61500, SDS-PAGE | Tricholoma matsutake |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| glucoamylase | - |
Tricholoma matsutake |
| TmGLA | - |
Tricholoma matsutake |
| TmGlu1 | - |
Tricholoma matsutake |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
recombinant enzyme | Tricholoma matsutake |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 75 | activity range, profile overview | Tricholoma matsutake |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 4 | 50 | purified enzyme, stable at | Tricholoma matsutake |
| 70 | - |
purified enzyme, inactivation | Tricholoma matsutake |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
recombinant enzyme | Tricholoma matsutake |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 2 | 6.5 | activity range, profile overview | Tricholoma matsutake |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 2 | - |
purified enzyme, complete loss activity | Tricholoma matsutake |
| 3 | - |
purified enzyme, loss of 80% activity | Tricholoma matsutake |
| 4 | 6 | purified enzyme, stable at | Tricholoma matsutake |
| 7 | - |
purified enzyme, loss of 60% activity | Tricholoma matsutake |
| 8 | - |
purified enzyme, complete loss activity | Tricholoma matsutake |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the enzyme belongs to the glycosyl hydrolase family 15, GH15 | Tricholoma matsutake |
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Release 2023.1 (January 2023)
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