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Literature summary for 3.2.1.3 extracted from

  • Ashly, P.; Mohanan, P.
    Preparation and characterization of Rhizopus amyloglucosidase immobilized on poly(o-toluidine) (2010), Process Biochem., 45, 1422-1426.
No PubMed abstract available
Search for more literature for 3.2.1.3

Protein Variants

Protein Variants Comment Organism
additional information immobilization of the enzyme onto chemically synthesized poly(o-toluidine) salt and base powders using adsorption and covalent crosslinking with glutaraldehyde, overview, The immobilized enzyme has a better thermal stability than the free enzyme Rhizopus sp.

Organism

Organism UniProt Comment Textmining
Rhizopus sp.
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 activities of the enzyme immobilized on different polymers, overview Rhizopus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
soluble starch + H2O
-
 Rhizopus sp. beta-D-glucose + ?
-
 ?

Synonyms

Synonyms Comment Organism
amyloglucosidase
-
 Rhizopus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
 free enzyme Rhizopus sp.
65
-
 immobilized enzyme Rhizopus sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 60 activity range, immobilized enzyme Rhizopus sp.
10 50 activity range, free enzyme Rhizopus sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 60 min, 45% of activity of the free enzyme is remaining, and 70-95% of the immobilized enzyme Rhizopus sp.
70
-
 60 min, loss of activity of the free enzyme, and 60-90% activity remaining of the immobilized enzyme Rhizopus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
 free and immobilized enzyme Rhizopus sp.

pH Range

pH Minimum pH Maximum Comment Organism
4 7 activity range both free and immobilized enzyme Rhizopus sp.