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Literature summary for 3.2.1.3 extracted from
- Influence of different substrates on the production of a mutant thermostable glucoamylase in submerged fermentation (2011), Appl. Biochem. Biotechnol., 163, 14-24.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of wild-type and mutant S54P/T314A/H415Y enzymes in Saccharomyces cerevisiae | Aspergillus awamori |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| S54P/T314A/H415Y | the mutant enzyme is more thermostable compared to the wild-type enzyme at 70°C. The mutation does not affect the protein secretion nor the production of the enzyme | Aspergillus awamori |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 100000 | - |
mutant S54P/T314A/H415Y | Aspergillus awamori |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus awamori | - |
- |
- |
| Aspergillus awamori C468 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme mutant S54P/T314A/H415Y from Saccharomyces cerevisiae by acarbose affinity chromatography | Aspergillus awamori |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| glucoamylase | - |
Aspergillus awamori |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
mutant S54P/T314A/H415Y | Aspergillus awamori |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
purified recombinant enzyme mutant S54P/T314A/H415Y, stable up to, irreversible thermo-inactivation of the mutant enzyme follows first-order kinetics | Aspergillus awamori |
| 65 | - |
purified recombinant enzyme mutant S54P/T314A/H415Y, half-life is 76 min | Aspergillus awamori |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
mutant S54P/T314A/H415Y | Aspergillus awamori |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 9 | the recombinant enzyme mutant S54P/T314A/H415Y retains 80% of its activity within this range | Aspergillus awamori |
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Release 2023.1 (January 2023)
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