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Literature summary for 3.2.1.3 extracted from
- Cloning, recombinant expression and characterization of a new glucoamylase gene from Aureobasidium pullulans NRRL 12974 and its potential application in raw potato starch degradation (2011), Afr. J. Biotechnol., 10, 9122-9131.
No PubMed abstract available
Application
| Application | Comment | Organism |
|---|---|---|
| industry | this glucoamylase may find important applications in the starch saccharification industry and in bioethanol production | Aureobasidium pullulans |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene APGA1, DNA and amino acid sequence determination and analysis, expression in Pichia pastoris | Aureobasidium pullulans |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Aureobasidium pullulans | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 66000 | - |
x * 66000, SDS-PAGE | Aureobasidium pullulans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aureobasidium pullulans | E2GDF4 | gene APGA1 | - |
| Aureobasidium pullulans NRRL 12974 | E2GDF4 | gene APGA1 | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Aureobasidium pullulans |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from Pichia pastoris by ultrafiltration, gel filtration, and anion exchange chromatography to homogeneity | Aureobasidium pullulans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 298 | - |
purified recombinant enzyme, pH 4.5, 60°C, substarte raw potato starch | Aureobasidium pullulans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylopectin + H2O | - |
Aureobasidium pullulans | ? | - |
? |  |
| amylopectin + H2O | - |
Aureobasidium pullulans NRRL 12974 | ? | - |
? | |
| amylose + H2O | - |
Aureobasidium pullulans | ? | - |
? | |
| amylose + H2O | - |
Aureobasidium pullulans NRRL 12974 | ? | - |
? | |
| glycogen + H2O | - |
Aureobasidium pullulans | ? | - |
? | |
| glycogen + H2O | - |
Aureobasidium pullulans NRRL 12974 | ? | - |
? | |
| additional information | poor activity with pullulan and laminarin | Aureobasidium pullulans | ? | - |
? | |
| additional information | poor activity with pullulan and laminarin | Aureobasidium pullulans NRRL 12974 | ? | - |
? | |
| raw potato starch + H2O | - |
Aureobasidium pullulans | ? | - |
? | |
| soluble starch + H2O | - |
Aureobasidium pullulans | ? | - |
? | |
| soluble starch + H2O | - |
Aureobasidium pullulans NRRL 12974 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 66000, SDS-PAGE | Aureobasidium pullulans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| APGA1 | - |
Aureobasidium pullulans |
| glucoamylase | - |
Aureobasidium pullulans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Aureobasidium pullulans |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 80 | activity range | Aureobasidium pullulans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
- |
Aureobasidium pullulans |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 6 | purified recombinant enzyme, stable between pH 3 and pH 6. The catalytic activity of the enzyme is reduced quickly at higher than pH 5 and is totally lost at higher than pH 7 | Aureobasidium pullulans |
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