Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Caldanaerobacter subterraneus subsp. tengcongensis MB4 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | - |
Caldanaerobacter subterraneus subsp. tengcongensis MB4 | |
EDTA | - |
Caldanaerobacter subterraneus subsp. tengcongensis MB4 | |
Pb2+ | - |
Caldanaerobacter subterraneus subsp. tengcongensis MB4 | |
Zn2+ | - |
Caldanaerobacter subterraneus subsp. tengcongensis MB4 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.35 | - |
maltotetraose | pH 5.0, 75°C | Caldanaerobacter subterraneus subsp. tengcongensis MB4 | |
1.63 | - |
maltopentaose | pH 5.0, 75°C | Caldanaerobacter subterraneus subsp. tengcongensis MB4 | |
1.88 | - |
maltohexaose | pH 5.0, 75°C | Caldanaerobacter subterraneus subsp. tengcongensis MB4 | |
1.93 | - |
maltotriose | pH 5.0, 75°C | Caldanaerobacter subterraneus subsp. tengcongensis MB4 | |
2.21 | - |
maltoheptaose | pH 5.0, 75°C | Caldanaerobacter subterraneus subsp. tengcongensis MB4 | |
2.41 | - |
isomaltose | pH 5.0, 75°C | Caldanaerobacter subterraneus subsp. tengcongensis MB4 | |
13.4 | - |
maltose | pH 5.0, 75°C | Caldanaerobacter subterraneus subsp. tengcongensis MB4 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | slight increase in enzyme activtiy | Caldanaerobacter subterraneus subsp. tengcongensis MB4 | |
Mn2+ | slight increase in enzyme activity | Caldanaerobacter subterraneus subsp. tengcongensis MB4 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
77000 | - |
- |
Caldanaerobacter subterraneus subsp. tengcongensis MB4 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Caldanaerobacter subterraneus subsp. tengcongensis MB4 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partially purification to 30fold homogeneity by heat treatment and gel filtration chromatography | Caldanaerobacter subterraneus subsp. tengcongensis MB4 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
80 | - |
- |
Caldanaerobacter subterraneus subsp. tengcongensis MB4 |
175 | - |
after purification | Caldanaerobacter subterraneus subsp. tengcongensis MB4 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
isomaltose + H2O | assay at pH 5.0, 75°C, reaction stopped by boiling at 100°C for 3 min | Caldanaerobacter subterraneus subsp. tengcongensis MB4 | D-glucose + ? | - |
? | |
maltoheptaose + H2O | assay at pH 5.0, 75°C, reaction stopped by boiling at 100°C for 3 min | Caldanaerobacter subterraneus subsp. tengcongensis MB4 | D-glucose + ? | - |
? | |
maltohexaose + H2O | assay at pH 5.0, 75°C, reaction stopped by boiling at 100°C for 3 min | Caldanaerobacter subterraneus subsp. tengcongensis MB4 | D-glucose + ? | - |
? | |
maltopentaose + H2O | assay at pH 5.0, 75°C, reaction stopped by boiling at 100°C for 3 min | Caldanaerobacter subterraneus subsp. tengcongensis MB4 | D-glucose + ? | - |
? | |
maltose + H2O | assay at pH 5.0, 75°C, reaction stopped by boiling at 100°C for 3 min | Caldanaerobacter subterraneus subsp. tengcongensis MB4 | D-glucose + ? | - |
? | |
maltotetraose + H2O | assay at pH 5.0, 75°C, reaction stopped by boiling at 100°C for 3 min | Caldanaerobacter subterraneus subsp. tengcongensis MB4 | D-glucose + ? | - |
? | |
maltotriose + H2O | assay at pH 5.0, 75°C, reaction stopped by boiling at 100°C for 3 min | Caldanaerobacter subterraneus subsp. tengcongensis MB4 | D-glucose + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Caldanaerobacter subterraneus subsp. tengcongensis MB4 |
Synonyms | Comment | Organism |
---|---|---|
glucoamylase | - |
Caldanaerobacter subterraneus subsp. tengcongensis MB4 |
TtcGA | - |
Caldanaerobacter subterraneus subsp. tengcongensis MB4 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
- |
Caldanaerobacter subterraneus subsp. tengcongensis MB4 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
no activity loss after incubation at 75°C for 6 h, half life at 80°C 2.6 h | Caldanaerobacter subterraneus subsp. tengcongensis MB4 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10.7 | - |
isomaltose | pH 5.0, 75°C | Caldanaerobacter subterraneus subsp. tengcongensis MB4 | |
149 | - |
maltose | pH 5.0, 75°C | Caldanaerobacter subterraneus subsp. tengcongensis MB4 | |
343 | - |
maltoheptaose | pH 5.0, 75°C | Caldanaerobacter subterraneus subsp. tengcongensis MB4 | |
368 | - |
maltotriose | pH 5.0, 75°C | Caldanaerobacter subterraneus subsp. tengcongensis MB4 | |
459 | - |
maltohexaose | pH 5.0, 75°C | Caldanaerobacter subterraneus subsp. tengcongensis MB4 | |
460 | - |
maltotetraose | pH 5.0, 75°C | Caldanaerobacter subterraneus subsp. tengcongensis MB4 | |
518 | - |
maltopentaose | pH 5.0, 75°C | Caldanaerobacter subterraneus subsp. tengcongensis MB4 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Caldanaerobacter subterraneus subsp. tengcongensis MB4 |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 7 | above and below abruptly loss of enzyme activity | Caldanaerobacter subterraneus subsp. tengcongensis MB4 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.44 | - |
isomaltose | pH 5.0, 75°C | Caldanaerobacter subterraneus subsp. tengcongensis MB4 | |
11.1 | - |
maltose | pH 5.0, 75°C | Caldanaerobacter subterraneus subsp. tengcongensis MB4 | |
155 | - |
maltoheptaose | pH 5.0, 75°C | Caldanaerobacter subterraneus subsp. tengcongensis MB4 | |
191 | - |
maltotriose | pH 5.0, 75°C | Caldanaerobacter subterraneus subsp. tengcongensis MB4 | |
244 | - |
maltohexaose | pH 5.0, 75°C | Caldanaerobacter subterraneus subsp. tengcongensis MB4 | |
318 | - |
maltopentaose | pH 5.0, 75°C | Caldanaerobacter subterraneus subsp. tengcongensis MB4 | |
341 | - |
maltotetraose | pH 5.0, 75°C | Caldanaerobacter subterraneus subsp. tengcongensis MB4 |