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Literature summary for 3.2.1.3 extracted from

  • Bhatti, H.N.; Rashid, M.H.; Asgher, M.; Nawaz, R.; Khalid, A.M.; Perveen, R.
    Chemical modification results in hyperactivation and thermostabilization of Fusarium solani glucoamylase (2007), Can. J. Microbiol., 53, 177-185.
    View publication on PubMed
Search for more literature for 3.2.1.3

Activating Compound

Activating Compound Comment Organism Structure
EDTA chemical modification of the enzyme's carboxyl groups with EDTA as nucleophile in presence of 1-ethyl-3(3-dimethylaminopropyl)carbodiimide leads to a dramatic enhancement of enzyme catalytic activity and thermal stability Fusarium solani

General Stability

General Stability Organism
the EDTA-coupled enzyme is more stable towards proteases and urea Fusarium solani

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics and thermodynamics of native and EDTA-coupled enzyme, overview Fusarium solani
1.6
-
 starch pH 5.5, 40°C, native enzyme Fusarium solani
2.3 2.9 starch pH 5.5, 40°C, EDTA-coupled enzyme Fusarium solani

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
starch + H2O Fusarium solani
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Fusarium solani
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme to homogeneity by anion exchange and hydrophobic interaction chromatography, and gel filtration Fusarium solani

Source Tissue

Source Tissue Comment Organism Textmining
additional information solid-state fermentation on wheat bran Fusarium solani
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Fusarium solani

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
starch + H2O
-
 Fusarium solani ?
-
 ?
starch + H2O soluble starch Fusarium solani ?
-
 ?

Synonyms

Synonyms Comment Organism
glucoamylase
-
 Fusarium solani

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 assay at Fusarium solani

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 75
-
 Fusarium solani

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 half-life of native enzyme is 128 min Fusarium solani
60
-
 half-life of native enzyme is 25 min Fusarium solani

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
217
-
 starch pH 5.5, 40°C, native enzyme Fusarium solani
485 518 starch pH 5.5, 40°C, EDTA-coupled enzyme Fusarium solani

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
 native enzyme Fusarium solani
5 6 EDTA-coupled enzyme Fusarium solani

pH Range

pH Minimum pH Maximum Comment Organism
2 9
-
 Fusarium solani