Application | Comment | Organism |
---|---|---|
biotechnology | the enzyme from commercial preparation is immobilized by sorption on a carbon support Sibunit, starch and dextrin hydrolysis kinetic parameters of glucoamylase, including the rate constant of thermal inactivation, show that immobilization of the enzyme results in a 1000fold increase in enzyme stability in comparison to the dissolved enzyme, presence of the dextrin substrate has a stabilizing effect, increase in dextrin concentration to 53% increases the thermostability of the immobilized enzyme, the immobilized-enzyme biocatalyst for starch saccharification has a high operational stability, half-inactivation time at 60°C exceeds 30 days | Aspergillus awamori |
Protein Variants | Comment | Organism |
---|---|---|
additional information | the enzyme from commercial preparation is immobilized by sorption on a carbon support Sibunit, starch and dextrin hydrolysis kinetic parameters of glucoamylase, including the rate constant of thermal inactivation, show that immobilization of the enzyme results in a 1000fold increase in enzyme stability in comparison to the dissolved enzyme, presence of the dextrin substrate has a stabilizing effect, increase in dextrin concentration to 53% increases the thermostability of the immobilized enzyme, the immobilized-enzyme biocatalyst for starch saccharification has a high operational stability, half-inactivation time at 60°C exceeds 30 days, method optimization, overview | Aspergillus awamori |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dextrin + 6 H2O | Aspergillus awamori | the enzyme performs hydrolytic cleavage of terminal alpha-glycosyl residues from starch and dextrin molecules | 7 D-glucose | - |
? | |
starch + H2O | Aspergillus awamori | the enzyme performs hydrolytic cleavage of terminal alpha-glycosyl residues from starch and dextrin molecules | D-glucose + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus awamori | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Aspergillus awamori |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
commercial preparation | - |
Aspergillus awamori | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
200 | - |
purified commercial preparation | Aspergillus awamori |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dextrin + 6 H2O | the enzyme performs hydrolytic cleavage of terminal alpha-glycosyl residues from starch and dextrin molecules | Aspergillus awamori | 7 D-glucose | - |
? | |
dextrin + 6 H2O | potato dextrin, the enzyme performs hydrolytic cleavage of terminal alpha-glycosyl residues from starch and dextrin molecules | Aspergillus awamori | 7 D-glucose | - |
? | |
starch + H2O | the enzyme performs hydrolytic cleavage of terminal alpha-glycosyl residues from starch and dextrin molecules | Aspergillus awamori | D-glucose + ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
exo-1,4-alpha-glucosidase | - |
Aspergillus awamori |
glucoamylase | - |
Aspergillus awamori |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Aspergillus awamori |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 80 | - |
Aspergillus awamori |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.6 | - |
- |
Aspergillus awamori |