Literature summary for 3.2.1.3 extracted from

Adam, A.C.; Latorre-Garcia, L.; Polaina, J.
Structural analysis of glucoamylase encoded by the STA1 gene of Saccharomyces cerevisiae (var. diastaticus) (2004), Yeast, 21, 379-388.

Search for more literature for 3.2.1.3

Cloned(Commentary)

Cloned (Comment)	Organism
gene STA1, DNA and amino acid sequence determination and analysis, homology modelling	Saccharomyces cerevisiae 'var. diastaticus'

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
extracellular	the enzyme is secreted from the cells	Saccharomyces cerevisiae 'var. diastaticus'	-	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
80000	-	x * 80000, SDS-PAGE	Saccharomyces cerevisiae 'var. diastaticus'
300000	-	native PAGE	Saccharomyces cerevisiae 'var. diastaticus'

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
starch + H2O	Saccharomyces cerevisiae 'var. diastaticus'	soluble starch	starch + beta-D-glucose	-	?

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae 'var. diastaticus'	P04065	gene STA1, different strains	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
glycoprotein	Sta1p is hyperglycosylized, over 80% carbohydrate content	Saccharomyces cerevisiae 'var. diastaticus'

Purification (Commentary)

Purification (Comment)	Organism
partially	Saccharomyces cerevisiae 'var. diastaticus'

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	activity in different strains	Saccharomyces cerevisiae 'var. diastaticus'

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	the enzyme binds poorly to insoluble starch	Saccharomyces cerevisiae 'var. diastaticus'	?	-	?
starch + H2O	soluble starch	Saccharomyces cerevisiae 'var. diastaticus'	starch + beta-D-glucose	-	?

Subunits

Subunits	Comment	Organism
More	structure analysis of Sta1p, modelling	Saccharomyces cerevisiae 'var. diastaticus'
oligomer	x * 80000, SDS-PAGE	Saccharomyces cerevisiae 'var. diastaticus'

Synonyms

Synonyms	Comment	Organism
glucoamylase	-	Saccharomyces cerevisiae 'var. diastaticus'

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Release 2023.1 (January 2023)