Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 60% inhibition | Bacillus sp. (in: Bacteria) | |
Cu2+ | complete inhibition | Bacillus sp. (in: Bacteria) | |
Hg2+ | complete inhibition | Bacillus sp. (in: Bacteria) | |
K+ | complete inhibition | Bacillus sp. (in: Bacteria) |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | activates | Bacillus sp. (in: Bacteria) | |
Fe2+ | activates | Bacillus sp. (in: Bacteria) | |
Mg2+ | activates | Bacillus sp. (in: Bacteria) |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | - |
- |
- |
Bacillus sp. (in: Bacteria) J38 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme partially 10.2fold by ammonium sulfate fractionation, gel filtration, and ion exchange chromatography | Bacillus sp. (in: Bacteria) |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(alpha-D-glucopyranosyl-(1-4))n-alpha-D-glucopyranose + H2O = (alpha-D-glucopyranosyl-(1-4))n-1-alpha-D-glucopyranose + beta-D-glucopyranose | mode of action | Bacillus sp. (in: Bacteria) |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | maximal enzyme expression at 65°C and pH 7.0 | Bacillus sp. (in: Bacteria) | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
34 | - |
partially purified enzyme | Bacillus sp. (in: Bacteria) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
maltose + H2O | - |
Bacillus sp. (in: Bacteria) | beta-D-glucose + D-glucose | product analysis | ? | |
maltose + H2O | - |
Bacillus sp. (in: Bacteria) J38 | beta-D-glucose + D-glucose | product analysis | ? | |
starch + H2O | - |
Bacillus sp. (in: Bacteria) | starch + beta-D-glucose | product analysis | ? | |
starch + H2O | - |
Bacillus sp. (in: Bacteria) J38 | starch + beta-D-glucose | product analysis | ? |
Synonyms | Comment | Organism |
---|---|---|
GA | - |
Bacillus sp. (in: Bacteria) |
glucoamylase | - |
Bacillus sp. (in: Bacteria) |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
at pH 5.0 | Bacillus sp. (in: Bacteria) |
80 | - |
at pH 7.0 | Bacillus sp. (in: Bacteria) |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 90 | - |
Bacillus sp. (in: Bacteria) |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
stability profile | Bacillus sp. (in: Bacteria) |
60 | - |
pH 7.0, half-life is 13 h | Bacillus sp. (in: Bacteria) |
65 | - |
pH 7.0, half-life is 8 h | Bacillus sp. (in: Bacteria) |
70 | - |
pH 7.0, half-life is 40 min | Bacillus sp. (in: Bacteria) |
80 | - |
stable up to for 30 min at pH 7.0, loss of 40% activity at pH 5.0 | Bacillus sp. (in: Bacteria) |
100 | - |
36% remaining activity after 10 min at pH 7.0, loss of 89% activity at pH 5.0 | Bacillus sp. (in: Bacteria) |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Bacillus sp. (in: Bacteria) |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 8 | - |
Bacillus sp. (in: Bacteria) |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
stability profile | Bacillus sp. (in: Bacteria) |
3 | 10 | maximally stable at pH 7.0 | Bacillus sp. (in: Bacteria) |