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Literature summary for 3.2.1.3 extracted from
- Purification and characterization of a thermostable glucoamylase from Chaetomium thermophilum (2005), J. Gen. Appl. Microbiol., 51, 175-181.
Application
| Application | Comment | Organism |
|---|---|---|
| biotechnology | the enzyme might be useful in biotechnological processes | Thermochaetoides thermophila |
| industry | the enzyme is used in industrial starch degradation | Thermochaetoides thermophila |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | complete inhibition at 1 mM | Thermochaetoides thermophila |  |
| Cu2+ | 12% inhibition at 1 mM | Thermochaetoides thermophila | |
| Fe2+ | 80% inhibition at 1 mM | Thermochaetoides thermophila | |
| Hg2+ | complete inhibition at 1 mM | Thermochaetoides thermophila | |
| Mn2+ | 11% inhibition at 1 mM | Thermochaetoides thermophila | |
| additional information | poor inhibition by Ba2+ at 1 mM | Thermochaetoides thermophila | |
| Zn2+ | 25% inhibition at 1 mM | Thermochaetoides thermophila | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | the enzyme is secreted to the culture medium | Thermochaetoides thermophila | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 20% activation at 1 mM | Thermochaetoides thermophila | |
| K+ | 10% activation at 1 mM | Thermochaetoides thermophila | |
| additional information | no effect by Mg2+ at 1 mM | Thermochaetoides thermophila | |
| Na+ | 12% activation at 1 mM | Thermochaetoides thermophila | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 64000 | - |
1 * 64000, SDS-PAGE | Thermochaetoides thermophila |
| 65400 | - |
gel filtration and native PAGE | Thermochaetoides thermophila |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermochaetoides thermophila | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native soluble enzyme from culture medium 16.3 fold to homogeneity by ammonium sulfate fractionation, ion exchange and hydrophobic interaction chromatography, and gel filtration | Thermochaetoides thermophila |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 71.2 | - |
purified enzyme | Thermochaetoides thermophila |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| starch + H2O | - |
Thermochaetoides thermophila | starch + beta-D-glucose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 64000, SDS-PAGE | Thermochaetoides thermophila |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| exo-1,4-alpha-D-glucanohydrolase | - |
Thermochaetoides thermophila |
| glucoamylase | - |
Thermochaetoides thermophila |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
- |
Thermochaetoides thermophila |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 60 | stable at, purified enzyme | Thermochaetoides thermophila |
| 65 | - |
60 min, 50% activity remaining of purified enzyme | Thermochaetoides thermophila |
| 70 | - |
30 min, 40% activity remaining of purified enzyme | Thermochaetoides thermophila |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
- |
Thermochaetoides thermophila |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 2.5 | 9 | 30% of maximal activity at pH 3.0 and pH 8.5, 50% of maximal activity at pH 3.5 and pH 7.0 | Thermochaetoides thermophila |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | 8 | stable at pH 3.0-8.0, about 50% remaining activity at pH 3.0 and pH 10.0, 30% at pH 2.5 and pH 11.0 | Thermochaetoides thermophila |
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