Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-chloromercuribenzoate | complete inhibition at 10 mM, 72% inhibition at 1 mM | Camelus dromedarius | |
Co2+ | 26% inhibition at 1 mM | Camelus dromedarius | |
Cu2+ | 85% inhibition at 1 mM | Camelus dromedarius | |
DTNB | 42% inhibition at 10 mM, no inhibition at 1 mM | Camelus dromedarius | |
Hg2+ | 89% inhibition at 1 mM | Camelus dromedarius | |
iodoacetamide | 78% inhibition at 10 mM, 40% inhibition at 1 mM | Camelus dromedarius | |
additional information | no inhibition by PMSF | Camelus dromedarius | |
Zn2+ | 78% inhibition at 1 mM | Camelus dromedarius |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10 | - |
starch | pH 5.5, 37°C | Camelus dromedarius |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | the enzyme is secreted by the cells in pancreas to small intestine | Camelus dromedarius | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | 65% activation at 1 mM | Camelus dromedarius | |
Ca2+ | 74% activation at 1 mM | Camelus dromedarius | |
Mn2+ | 60% activation at 1 mM | Camelus dromedarius | |
Ni2+ | 11% activation at 1 mM | Camelus dromedarius |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58000 | - |
gel filtration | Camelus dromedarius |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Camelus dromedarius | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 93fold from pancreas to homogeneity by glycogen precipitation, ion exchange chromatography, and gel filtration | Camelus dromedarius |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
pancreas | - |
Camelus dromedarius | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.02 | - |
pancreas | Camelus dromedarius |
16.25 | - |
purified enzyme | Camelus dromedarius |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-cyclodextrin + H2O | - |
Camelus dromedarius | alpha-cyclodextrin + beta-D-glucose | - |
? | |
amylopectin + H2O | - |
Camelus dromedarius | amylopectin + beta-D-glucose | - |
? | |
dextrin + H2O | - |
Camelus dromedarius | dextrin + beta-D-glucose | - |
? | |
glycogen + H2O | - |
Camelus dromedarius | glycogen + beta-D-glucose | - |
? | |
additional information | substrate specificity overview | Camelus dromedarius | ? | - |
? | |
pectin + H2O | - |
Camelus dromedarius | pectin + beta-D-glucose | - |
? | |
starch + H2O | soluble potato starch | Camelus dromedarius | starch + beta-D-glucose | - |
? | |
xylan + H2O | - |
Camelus dromedarius | xylan + beta-D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 58000, SDS-PAGE | Camelus dromedarius |
Synonyms | Comment | Organism |
---|---|---|
glucoamylase | - |
Camelus dromedarius |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Camelus dromedarius |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
stable up to | Camelus dromedarius |
60 | - |
inactivation | Camelus dromedarius |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Camelus dromedarius |