Any feedback?
Literature summary for 3.2.1.3 extracted from
- Identification and characterization of glucoamylase from the fungus Thermomyces lanuginosus (2006), Biochim. Biophys. Acta, 1764, 671-676.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene gla1, DNA and amino acid sequence determination and analysis, expression in Pichia pastoris, phylogenetic analysis | Thermomyces lanuginosus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.5 | - |
maltose | pH 5.0, 70°C | Thermomyces lanuginosus |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 66000 | - |
x * 66000, SDS-PAGE | Thermomyces lanuginosus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermomyces lanuginosus | Q58HN1 | precursor; gene gla1 | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylopectin + H2O | 94% of activity with amylose | Thermomyces lanuginosus | amylopectin + beta-D-glucose | - |
? | |
| amylose + H2O | preferred substrate | Thermomyces lanuginosus | amylose + beta-D-glucose | - |
? | |
| glycogen + H2O | 87% of activity with amylose | Thermomyces lanuginosus | glycogen + beta-D-glucose | - |
? | |
| maltose + H2O | 14% of activity with amylose | Thermomyces lanuginosus | beta-D-glucose + D-glucose | - |
? | |
| maltotriose + H2O | 40% of activity with amylose | Thermomyces lanuginosus | maltose + beta-D-glucose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 66000, SDS-PAGE | Thermomyces lanuginosus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GA | - |
Thermomyces lanuginosus |
| glucoamylase | - |
Thermomyces lanuginosus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
- |
Thermomyces lanuginosus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 25.3 | - |
maltose | pH 5.0, 70°C | Thermomyces lanuginosus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Thermomyces lanuginosus |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Thermomyces lanuginosus | isoelectric focusing | - |
3.84 |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
