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Literature summary for 3.2.1.3 extracted from

  • Kim, M.S.; Park, J.T.; Kim, Y.W.; Lee, H.S.; Nyawira, R.; Shin, H.S.; Park, C.S.; Yoo, S.H.; Kim, Y.R.; Moon, T.W.; Park, K.H.
    Properties of a novel thermostable glucoamylase from the hyperthermophilic archaeon Sulfolobus solfataricus in relation to starch processing (2004), Appl. Environ. Microbiol., 70, 3933-3940.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
biotechnology the enzyme is potentially useful in improvement of industrial starch processing by eliminating the need to adjust both pH and temperature Saccharolobus solfataricus

Cloned(Commentary)

Cloned (Comment) Organism
gene ssg, phylogenetic analysis, expression in Escherichia coli Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus Q97ZD0 gene ssg
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme 80.9fold from Escherichia coli by heat treatment at 70°C, hydrophobic interaction chromatography, and gel filtration Saccharolobus solfataricus

Reaction

Reaction Comment Organism Reaction ID
(alpha-D-glucopyranosyl-(1-4))n-alpha-D-glucopyranose + H2O = (alpha-D-glucopyranosyl-(1-4))n-1-alpha-D-glucopyranose + beta-D-glucopyranose reaction mechanism depends on the substrate, i.e. hydrolysis or transglycosylation, overview Saccharolobus solfataricus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
56.3
-
purified recombinant enzyme Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
maltooligomers + H2O substrate specificity Saccharolobus solfataricus ? + beta-D-glucose
-
r
maltotriose + H2O exoglycosidic hydrolysis of terminal glucose in forward reaction, preferred substrate, synthesis of isomaltose from beta-D-glucose in the reverse reaction with low activity Saccharolobus solfataricus maltose + beta-D-glucose anomeric product configuration analysis at 80°C r
additional information bifunctional enzyme performing hydrolysis or transglycosylation depending on the substrate Saccharolobus solfataricus ?
-
?

Subunits

Subunits Comment Organism
tetramer
-
Saccharolobus solfataricus

Synonyms

Synonyms Comment Organism
glucoamylase
-
Saccharolobus solfataricus
SSG
-
Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
90
-
-
Saccharolobus solfataricus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
60 100 90% of maximal activity at 80°C, low activity at 100°C Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
additional information substrate specificity Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
Saccharolobus solfataricus

pH Range

pH Minimum pH Maximum Comment Organism
4 8
-
Saccharolobus solfataricus