Cloned (Comment) | Organism |
---|---|
expression in Aspergillus niger | Rasamsonia emersonii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.61 | - |
maltoheptaose | 45°C, pH 4.5 | Rasamsonia emersonii | |
3.8 | - |
maltose | 45°C, pH 4.5 | Rasamsonia emersonii | |
53.6 | - |
isomaltose | 45°C, pH 4.5 | Rasamsonia emersonii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
62800 | - |
x * 62800, deduced from nucleotide sequence | Rasamsonia emersonii |
70000 | - |
x * 70000, SDS-PAGE, recombinant glucoamylase | Rasamsonia emersonii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
starch + H2O | Rasamsonia emersonii | - |
beta-D-glucose + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rasamsonia emersonii | Q9C1V4 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Rasamsonia emersonii |
Purification (Comment) | Organism |
---|---|
recombinant glucoamylase, ultrafiltration, SP-Sepharose, Q-Sepharose | Rasamsonia emersonii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
isomaltose + H2O | - |
Rasamsonia emersonii | beta-D-glucose | - |
r | |
maltoheptaose + H2O | - |
Rasamsonia emersonii | maltohexaose + D-glucose | - |
? | |
maltose + H2O | - |
Rasamsonia emersonii | beta-D-glucose + D-glucose | - |
r | |
starch + H2O | - |
Rasamsonia emersonii | beta-D-glucose + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 62800, deduced from nucleotide sequence | Rasamsonia emersonii |
? | x * 70000, SDS-PAGE, recombinant glucoamylase | Rasamsonia emersonii |
Synonyms | Comment | Organism |
---|---|---|
GA | - |
Rasamsonia emersonii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Rasamsonia emersonii |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 90 | approx. 15% of maximal activity at 25°C, approx. 20% of maximal activity a 90°C | Rasamsonia emersonii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
approx. 20% and 50% losss of activity after 20 h and 48 h, respectively, in the presence of 30% glucose | Rasamsonia emersonii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.7 | - |
isomaltose | 45°C, pH 4.5 | Rasamsonia emersonii | |
31.3 | - |
maltose | 45°C, pH 4.5 | Rasamsonia emersonii | |
221.3 | - |
maltoheptaose | 45°C, pH 4.5 | Rasamsonia emersonii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
- |
Rasamsonia emersonii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 7 | approx. 50% of maximal activity at pH 3.0, almost no activity at pH 7.0 | Rasamsonia emersonii |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Rasamsonia emersonii | - |
- |
3.5 |