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Literature summary for 3.2.1.3 extracted from
- Protein engineering to improve the thermostability of glucoamylase from Aspergillus awamori based on molecular dynamics simulations (2003), Protein Eng., 16, 19-25.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Saccharomyces cerevisae | Aspergillus awamori |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| A276C/S347C | 101% of wild-type kcat | Aspergillus awamori |
| A276C/S347C/S298C | 107% of wild-type kcat | Aspergillus awamori |
| E389M | 104% of wild-type kcat | Aspergillus awamori |
| G396A | 90% of wild-type kcat | Aspergillus awamori |
| G396A/G407A | 92% of wild-type kcat | Aspergillus awamori |
| G407A | 96% of wild-type kcat | Aspergillus awamori |
| H391M | 89% of wild-type kcat | Aspergillus awamori |
| S298C/L354C | 104% of wild-type kcat | Aspergillus awamori |
| S386L | 103% of wild-type kcat | Aspergillus awamori |
| S418L | 103% of wild-type kcat | Aspergillus awamori |
| T390L | 101% of wild-type kcat | Aspergillus awamori |
| T416L | 101% of wild-type kcat | Aspergillus awamori |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.78 | - |
maltose | 50°C, pH 4.5 | Aspergillus awamori |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus awamori | P69327 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant glucoamylase, acarbose affinity chromatography | Aspergillus awamori |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 21000 | - |
- |
Aspergillus awamori |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| maltose + H2O | - |
Aspergillus awamori | beta-D-glucose + D-glucose | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GA | - |
Aspergillus awamori |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 8.9 | - |
maltose | 50°C, pH 4.5 | Aspergillus awamori | |
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Release 2023.1 (January 2023)
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