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Literature summary for 3.2.1.3 extracted from

  • Kumar, S.; Satyanarayana, T.
    Purification and kinetics of a raw starch-hydrolyzing, thermostable, and neutral glucoamylase of the thermophilic mold Thermomucor indicae-seudaticae (2003), Biotechnol. Prog., 19, 936-944.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
beta-mercaptoethanol 5 mM, 89% inhibition Thermomucor indicae-seudaticae
dithiothreitol 5 mM, 79% inhibition Thermomucor indicae-seudaticae
EDTA 5 mM, 78% inhibition Thermomucor indicae-seudaticae
Hg2+ 5 mM, 53% inhibition Thermomucor indicae-seudaticae
N-bromosuccinimide 5 mM, 83% inhibition Thermomucor indicae-seudaticae
N-ethylmaleimide 5 mM, complete inhibition Thermomucor indicae-seudaticae
Ni2+ 5 mM, 74% inhibition Thermomucor indicae-seudaticae
Propylene glycol 2%, 47% inhibition Thermomucor indicae-seudaticae
sodium dodecylsulfate 2%, 76% inhibition Thermomucor indicae-seudaticae
Triton X-100 2%, 60% inhibition Thermomucor indicae-seudaticae
Tween 20 2%, 27% inhibition Thermomucor indicae-seudaticae
Tween 40 2%, 42% inhibition Thermomucor indicae-seudaticae
Tween 80 2%, 25% inhibition Thermomucor indicae-seudaticae
Zn2+ 5 mM, complete inhibition Thermomucor indicae-seudaticae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
starch 2.4 mg/ml, 60°C Thermomucor indicae-seudaticae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
x * 42000, SDS-PAGE Thermomucor indicae-seudaticae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
starch + H2O Thermomucor indicae-seudaticae strongest activity with soluble starch beta-D-glucose + ?
-
?

Organism

Organism UniProt Comment Textmining
Thermomucor indicae-seudaticae
-
thermophilic mold
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein 9.0-10.5% carbohydrate Thermomucor indicae-seudaticae

Purification (Commentary)

Purification (Comment) Organism
lyophilization, acetone precipitation, SP-Sepharose, Sephadex G-50 Thermomucor indicae-seudaticae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
134
-
-
Thermomucor indicae-seudaticae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
starch + H2O strongest activity with soluble starch Thermomucor indicae-seudaticae beta-D-glucose + ?
-
?

Subunits

Subunits Comment Organism
? x * 42000, SDS-PAGE Thermomucor indicae-seudaticae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Thermomucor indicae-seudaticae

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 100 approx. 30% of maximal activity at 30°C, approx. 70% of maimal activity at 100% Thermomucor indicae-seudaticae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
50% loss of activity after 12 h Thermomucor indicae-seudaticae
80
-
50% loss of activity after 7 h Thermomucor indicae-seudaticae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Thermomucor indicae-seudaticae

pH Range

pH Minimum pH Maximum Comment Organism
4 10 approx. 15% of maximal activity at pH 4.0, approx. 85% of maximal activity at pH 10.0 Thermomucor indicae-seudaticae

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 8 approx. 5% loss of activity after 2 h at pH 6.0, pH 7.0 and pH 8,0, approx. 45% loss of activity after 20 h at pH 6.0 and pH 7.0, 45% loss of activity after 7 h at pH 8.0 Thermomucor indicae-seudaticae

pI Value

Organism Comment pI Value Maximum pI Value
Thermomucor indicae-seudaticae native PAGE
-
6.9