Inhibitors | Comment | Organism | Structure |
---|---|---|---|
beta-mercaptoethanol | 5 mM, 89% inhibition | Thermomucor indicae-seudaticae | |
dithiothreitol | 5 mM, 79% inhibition | Thermomucor indicae-seudaticae | |
EDTA | 5 mM, 78% inhibition | Thermomucor indicae-seudaticae | |
Hg2+ | 5 mM, 53% inhibition | Thermomucor indicae-seudaticae | |
N-bromosuccinimide | 5 mM, 83% inhibition | Thermomucor indicae-seudaticae | |
N-ethylmaleimide | 5 mM, complete inhibition | Thermomucor indicae-seudaticae | |
Ni2+ | 5 mM, 74% inhibition | Thermomucor indicae-seudaticae | |
Propylene glycol | 2%, 47% inhibition | Thermomucor indicae-seudaticae | |
sodium dodecylsulfate | 2%, 76% inhibition | Thermomucor indicae-seudaticae | |
Triton X-100 | 2%, 60% inhibition | Thermomucor indicae-seudaticae | |
Tween 20 | 2%, 27% inhibition | Thermomucor indicae-seudaticae | |
Tween 40 | 2%, 42% inhibition | Thermomucor indicae-seudaticae | |
Tween 80 | 2%, 25% inhibition | Thermomucor indicae-seudaticae | |
Zn2+ | 5 mM, complete inhibition | Thermomucor indicae-seudaticae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
starch | 2.4 mg/ml, 60°C | Thermomucor indicae-seudaticae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
x * 42000, SDS-PAGE | Thermomucor indicae-seudaticae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
starch + H2O | Thermomucor indicae-seudaticae | strongest activity with soluble starch | beta-D-glucose + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermomucor indicae-seudaticae | - |
thermophilic mold | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | 9.0-10.5% carbohydrate | Thermomucor indicae-seudaticae |
Purification (Comment) | Organism |
---|---|
lyophilization, acetone precipitation, SP-Sepharose, Sephadex G-50 | Thermomucor indicae-seudaticae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
134 | - |
- |
Thermomucor indicae-seudaticae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
starch + H2O | strongest activity with soluble starch | Thermomucor indicae-seudaticae | beta-D-glucose + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 42000, SDS-PAGE | Thermomucor indicae-seudaticae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Thermomucor indicae-seudaticae |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 100 | approx. 30% of maximal activity at 30°C, approx. 70% of maimal activity at 100% | Thermomucor indicae-seudaticae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
50% loss of activity after 12 h | Thermomucor indicae-seudaticae |
80 | - |
50% loss of activity after 7 h | Thermomucor indicae-seudaticae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Thermomucor indicae-seudaticae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 10 | approx. 15% of maximal activity at pH 4.0, approx. 85% of maximal activity at pH 10.0 | Thermomucor indicae-seudaticae |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | approx. 5% loss of activity after 2 h at pH 6.0, pH 7.0 and pH 8,0, approx. 45% loss of activity after 20 h at pH 6.0 and pH 7.0, 45% loss of activity after 7 h at pH 8.0 | Thermomucor indicae-seudaticae |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Thermomucor indicae-seudaticae | native PAGE | - |
6.9 |