Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Guanidine-HCl | 1 M, 1156% increase in activity | Thermoplasma acidophilum | |
Na2WO4 | slight stimulation | Thermoplasma acidophilum | |
Pefabloc SC | 5 mM, 129% increase in activity | Thermoplasma acidophilum | |
Triton X-100 | 0.1%, 162% increase in activity | Thermoplasma acidophilum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
sodium dodecylsulfate | 3 mM, complete inhibition | Picrophilus oshimae | |
sodium dodecylsulfate | 3 mM, complete inhibition | Thermoplasma acidophilum | |
sodiumdodecylsulfate | 3 mM, complete inhibition | Picrophilus torridus | |
Urea | 5 M, complete inhibition | Picrophilus oshimae | |
Urea | 5 M, complete inhibition | Picrophilus torridus | |
Urea | 5 M, complete inhibition | Thermoplasma acidophilum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
CaCl2 | 5 mM, 10% increase in activity | Picrophilus torridus | |
CaCl2 | 5 mM, 10% increase in activity | Picrophilus oshimae | |
CuSO4 | 5 mM, 10% increase in activity | Picrophilus torridus | |
CuSO4 | 5 mM, 10% increase in activity | Picrophilus oshimae | |
EDTA | 5 mM, 10% increase in activity | Picrophilus oshimae | |
FeSO4 | 5 mM, 10% increase in activity | Picrophilus torridus | |
FeSO4 | 5 mM, 10% increase in activity | Picrophilus oshimae | |
MgCl2 | 5 mM, 10% increase in activity | Picrophilus torridus | |
MgCl2 | 5 mM, 10% increase in activity | Picrophilus oshimae | |
Na2MoO4 | 5 mM, 10% increase in activity | Picrophilus torridus | |
Na2MoO4 | 5 mM, 10% increase in activity | Picrophilus oshimae | |
NaCl | 5 mM, 10% increase in activity | Picrophilus torridus | |
NaCl | 5 mM, 10% increase in activity | Picrophilus oshimae | |
ZnCl2 | 5 mM, 10% increase in activity | Picrophilus torridus | |
ZnCl2 | 5 mM, 10% increase in activity | Picrophilus oshimae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
85000 | - |
x * 140000 + x * 85000, SDS-PAGE | Picrophilus oshimae |
90000 | - |
x * 133000 + x * 90000, SDS-PAGE | Picrophilus torridus |
95000 | - |
x * 141000 + x * 95000, SDS-PAGE | Thermoplasma acidophilum |
133000 | - |
x * 133000 + x * 90000, SDS-PAGE | Picrophilus torridus |
140000 | - |
x * 140000 + x * 85000, SDS-PAGE | Picrophilus oshimae |
141000 | - |
x * 141000 + x * 95000, SDS-PAGE | Thermoplasma acidophilum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4 glucose | Picrophilus torridus | reversion or condensation reactions are observed when glucoamylase is incubated with 10% glucose | glucooligosaccharide + 3 H2O | - |
r | |
4 glucose | Picrophilus oshimae | reversion or condensation reactions are observed when glucoamylase is incubated with 10% glucose | glucooligosaccharide + 3 H2O | - |
r | |
amylopectin + H2O | Picrophilus oshimae | 100% of activity with soluble starch from potato | beta-D-glucose + ? | - |
? | |
amylopectin + H2O | Thermoplasma acidophilum | 104% of activity with soluble starch from potato | beta-D-glucose + ? | - |
? | |
amylopectin + H2O | Picrophilus torridus | 104% of activity with soluble starch from potato | beta-D-glucose + ? | - |
? | |
amylose + H2O | Picrophilus oshimae | 60% of activity with soluble starch from potato | beta-D-glucose + ? | - |
? | |
amylose + H2O | Thermoplasma acidophilum | 63% of activity with soluble starch from potato | beta-D-glucose + ? | - |
? | |
amylose + H2O | Picrophilus torridus | 74% of activity with soluble starch from potato | beta-D-glucose + ? | - |
? | |
dextrin + 6 H2O | Thermoplasma acidophilum | 84% of activity with soluble starch from potato | 7 beta-D-glucose | - |
? | |
dextrin + 6 H2O | Picrophilus oshimae | 89% of activity with soluble starch from potato | 7 beta-D-glucose | - |
? | |
dextrin + 6 H2O | Picrophilus torridus | 91% of activity with soluble starch from potato | 7 beta-D-glucose | - |
? | |
glycogen + 3 H2O | Picrophilus torridus | 103% of activity with soluble starch from potato | 4 beta-D-glucose | - |
? | |
glycogen + 3 H2O | Picrophilus oshimae | 90% of activity with soluble starch from potato | 4 beta-D-glucose | - |
? | |
glycogen + 3 H2O | Thermoplasma acidophilum | 96% of activity with soluble starch from potato | 4 beta-D-glucose | - |
? | |
isomaltose | Picrophilus torridus | reversion or condensation reactions are observed when glucoamylase is incubated with 10% isomaltose | isomaltooligosaccharide | - |
r | |
isomaltose | Picrophilus oshimae | reversion or condensation reactions are observed when glucoamylase is incubated with 10% isomaltose | isomaltooligosaccharide | - |
r | |
maltooligosaccharide + H2O | Picrophilus torridus | 49% of activity with soluble starch from potato | beta-D-glucose | - |
? | |
maltooligosaccharide + H2O | Thermoplasma acidophilum | 54% of activity with soluble starch from potato | beta-D-glucose | - |
? | |
maltooligosaccharide + H2O | Picrophilus oshimae | 55% of activity with soluble starch from potato | beta-D-glucose | - |
? | |
maltose + H2O | Picrophilus torridus | 17% of activity with soluble starch from potato | beta-D-glucose + D-glucose | - |
? | |
maltose + H2O | Picrophilus oshimae | 2% of activity with soluble starch from potato | beta-D-glucose + D-glucose | - |
? | |
maltose + H2O | Thermoplasma acidophilum | 24% of activity with soluble starch from potato | beta-D-glucose + D-glucose | - |
? | |
starch + H2O | Picrophilus oshimae | maize starch, 105% of activity with soluble starch from potato | beta-D-glucose + ? | - |
? | |
starch + H2O | Thermoplasma acidophilum | maize starch, 109% of activity with soluble starch from potato | beta-D-glucose + ? | - |
? | |
starch + H2O | Picrophilus torridus | maize starch, 83% of activity with soluble starch from potato | beta-D-glucose + ? | - |
? | |
starch + H2O | Thermoplasma acidophilum | native starch from potato, 105% of activity with soluble starch from potato | beta-D-glucose + ? | - |
? | |
starch + H2O | Picrophilus oshimae | native starch from potato, 105% of activity with soluble starch from potato | beta-D-glucose + ? | - |
? | |
starch + H2O | Picrophilus torridus | native starch from potato, 92% of activity with soluble starch from potato | beta-D-glucose + ? | - |
? | |
starch + H2O | Picrophilus oshimae | rice starch, 110% of activity with soluble starch from potato | beta-D-glucose + ? | - |
? | |
starch + H2O | Thermoplasma acidophilum | rice starch, 123% of activity with soluble starch from potato | beta-D-glucose + ? | - |
? | |
starch + H2O | Picrophilus torridus | rice starch, 95% of activity with soluble starch from potato | beta-D-glucose + ? | - |
? | |
starch + H2O | Thermoplasma acidophilum | soluble starch from potato | beta-D-glucose + ? | - |
? | |
starch + H2O | Picrophilus torridus | soluble starch from potato | beta-D-glucose + ? | - |
? | |
starch + H2O | Picrophilus oshimae | soluble starch from potato | beta-D-glucose + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Picrophilus oshimae | - |
- |
- |
Picrophilus torridus | - |
- |
- |
Thermoplasma acidophilum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
starch affinity chromatography, partially purified | Picrophilus oshimae |
Superdex 200, Superose 12 | Thermoplasma acidophilum |
Superdex 200, Superose 12 | Picrophilus torridus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3 | 8 | - |
Thermoplasma acidophilum |
3 | 8 | - |
Picrophilus torridus |
28.5 | - |
- |
Picrophilus oshimae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4 glucose | reversion or condensation reactions are observed when glucoamylase is incubated with 10% glucose | Thermoplasma acidophilum | glucooligosaccharide + 3 H2O | - |
r | |
4 glucose | reversion or condensation reactions are observed when glucoamylase is incubated with 10% glucose | Picrophilus torridus | glucooligosaccharide + 3 H2O | - |
r | |
4 glucose | reversion or condensation reactions are observed when glucoamylase is incubated with 10% glucose | Picrophilus oshimae | glucooligosaccharide + 3 H2O | - |
r | |
amylopectin + H2O | 100% of activity with soluble starch from potato | Picrophilus oshimae | beta-D-glucose + ? | - |
? | |
amylopectin + H2O | 104% of activity with soluble starch from potato | Thermoplasma acidophilum | beta-D-glucose + ? | - |
? | |
amylopectin + H2O | 104% of activity with soluble starch from potato | Picrophilus torridus | beta-D-glucose + ? | - |
? | |
amylose + H2O | 60% of activity with soluble starch from potato | Picrophilus oshimae | beta-D-glucose + ? | - |
? | |
amylose + H2O | 63% of activity with soluble starch from potato | Thermoplasma acidophilum | beta-D-glucose + ? | - |
? | |
amylose + H2O | 74% of activity with soluble starch from potato | Picrophilus torridus | beta-D-glucose + ? | - |
? | |
dextrin + 6 H2O | 84% of activity with soluble starch from potato | Thermoplasma acidophilum | 7 beta-D-glucose | - |
? | |
dextrin + 6 H2O | 89% of activity with soluble starch from potato | Picrophilus oshimae | 7 beta-D-glucose | - |
? | |
dextrin + 6 H2O | 91% of activity with soluble starch from potato | Picrophilus torridus | 7 beta-D-glucose | - |
? | |
glycogen + 3 H2O | 103% of activity with soluble starch from potato | Picrophilus torridus | 4 beta-D-glucose | - |
? | |
glycogen + 3 H2O | 90% of activity with soluble starch from potato | Picrophilus oshimae | 4 beta-D-glucose | - |
? | |
glycogen + 3 H2O | 96% of activity with soluble starch from potato | Thermoplasma acidophilum | 4 beta-D-glucose | - |
? | |
isomaltose | reversion or condensation reactions are observed when glucoamylase is incubated with 10% isomaltose | Thermoplasma acidophilum | isomaltooligosaccharide | - |
r | |
isomaltose | reversion or condensation reactions are observed when glucoamylase is incubated with 10% isomaltose | Picrophilus torridus | isomaltooligosaccharide | - |
r | |
isomaltose | reversion or condensation reactions are observed when glucoamylase is incubated with 10% isomaltose | Picrophilus oshimae | isomaltooligosaccharide | - |
r | |
maltooligosaccharide + H2O | 49% of activity with soluble starch from potato | Picrophilus torridus | beta-D-glucose | - |
? | |
maltooligosaccharide + H2O | 54% of activity with soluble starch from potato | Thermoplasma acidophilum | beta-D-glucose | - |
? | |
maltooligosaccharide + H2O | 55% of activity with soluble starch from potato | Picrophilus oshimae | beta-D-glucose | - |
? | |
maltose + H2O | 17% of activity with soluble starch from potato | Picrophilus torridus | beta-D-glucose + D-glucose | - |
? | |
maltose + H2O | 2% of activity with soluble starch from potato | Picrophilus oshimae | beta-D-glucose + D-glucose | - |
? | |
maltose + H2O | 24% of activity with soluble starch from potato | Thermoplasma acidophilum | beta-D-glucose + D-glucose | - |
? | |
starch + H2O | maize starch, 105% of activity with soluble starch from potato | Picrophilus oshimae | beta-D-glucose + ? | - |
? | |
starch + H2O | maize starch, 109% of activity with soluble starch from potato | Thermoplasma acidophilum | beta-D-glucose + ? | - |
? | |
starch + H2O | maize starch, 83% of activity with soluble starch from potato | Picrophilus torridus | beta-D-glucose + ? | - |
? | |
starch + H2O | native starch from potato, 105% of activity with soluble starch from potato | Thermoplasma acidophilum | beta-D-glucose + ? | - |
? | |
starch + H2O | native starch from potato, 105% of activity with soluble starch from potato | Picrophilus oshimae | beta-D-glucose + ? | - |
? | |
starch + H2O | native starch from potato, 92% of activity with soluble starch from potato | Picrophilus torridus | beta-D-glucose + ? | - |
? | |
starch + H2O | rice starch, 110% of activity with soluble starch from potato | Picrophilus oshimae | beta-D-glucose + ? | - |
? | |
starch + H2O | rice starch, 123% of activity with soluble starch from potato | Thermoplasma acidophilum | beta-D-glucose + ? | - |
? | |
starch + H2O | rice starch, 95% of activity with soluble starch from potato | Picrophilus torridus | beta-D-glucose + ? | - |
? | |
starch + H2O | soluble starch from potato | Thermoplasma acidophilum | beta-D-glucose + ? | - |
? | |
starch + H2O | soluble starch from potato | Picrophilus torridus | beta-D-glucose + ? | - |
? | |
starch + H2O | soluble starch from potato | Picrophilus oshimae | beta-D-glucose + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 133000 + x * 90000, SDS-PAGE | Picrophilus torridus |
? | x * 140000 + x * 85000, SDS-PAGE | Picrophilus oshimae |
? | x * 141000 + x * 95000, SDS-PAGE | Thermoplasma acidophilum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
- |
Thermoplasma acidophilum |
90 | - |
- |
Picrophilus torridus |
90 | - |
- |
Picrophilus oshimae |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 100 | approx. 20% of maximal activity at 50°C and 100°C, respectively | Thermoplasma acidophilum |
50 | 100 | approx. 20% of maximal activity at 50°C and 100°C, respectively | Picrophilus torridus |
50 | 100 | approx. 20% of maximal activity at 50°C and 100°C, respectively | Picrophilus oshimae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
no loss of activity after 24 h, 90% loss of activity after 60 h | Thermoplasma acidophilum |
70 | - |
no loss of activity after 24 h, 90% loss of activity after 60 h | Picrophilus torridus |
70 | - |
no loss of activity after 24 h, 90% loss of activity after 60 h | Picrophilus oshimae |
80 | - |
25% loss of activity after 24 h | Picrophilus oshimae |
80 | - |
no loss of activity after 24 h, 90% loss of activity after 60 h | Thermoplasma acidophilum |
80 | - |
no loss of activity after 24 h, 90% loss of activity after 60 h | Picrophilus torridus |
90 | - |
no loss of activity after 12 h, 50% loss of activity after 24 h | Thermoplasma acidophilum |
90 | - |
no loss of activity after 12 h, 50% loss of activity after 24 h | Picrophilus torridus |
90 | - |
no loss of activity after 12 h, 50% loss of activity after 24 h | Picrophilus oshimae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
2 | - |
- |
Thermoplasma acidophilum |
2 | - |
- |
Picrophilus torridus |
2 | - |
- |
Picrophilus oshimae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
1 | 5 | approx. 50% of maximal activity at pH 1.0, almost no activity at pH 5.0 | Picrophilus oshimae |
1 | 5 | approx. 60% of maximal activity at pH 1.0, almost no activity at pH 5.0 | Picrophilus torridus |
1 | 5 | approx. 70% of maximal activity at pH 1.0, almost no activity at pH 5.0 | Thermoplasma acidophilum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
1.4 | - |
no loss of activity after 6 h at 70°C | Thermoplasma acidophilum |
1.4 | - |
no loss of activity after 6 h at 70°C | Picrophilus torridus |
1.4 | - |
no loss of activity after 6 h at 70°C | Picrophilus oshimae |
7 | - |
39% loss of activity after 6 h at 70°C | Picrophilus torridus |
7 | - |
64% loss of activity after 6 h at 70°C | Thermoplasma acidophilum |
7 | - |
79% loss of activity after 6 h at 70°C | Picrophilus oshimae |
8 | - |
77% loss of activity after 6 h at 70°C | Picrophilus torridus |
8 | - |
complete loss of activity after 6 h at 70°C | Thermoplasma acidophilum |
8 | - |
complete loss of activity after 6 h at 70°C | Picrophilus oshimae |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Picrophilus torridus | isoelectric focusing | - |
4.5 |
Thermoplasma acidophilum | isoelectric focusing | - |
4.9 |
Picrophilus oshimae | isoelectric focusing | - |
4.9 |