KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | 6.57 mg/ml for glucoamylase I with starch as substrate, 4.52 mg/ml for glucoamylase II with starch as substrate. 0.15 mg/ml for glucoamylase II with alpha-cyclodextrin as substrate, 2.0 mg/ml for glucoamylase II with beta-cyclodextrin as substrate | Lipomyces kononenkoae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Lipomyces kononenkoae | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
128000 | - |
1 * 128000, glucoamylase II, SDS-PAGE | Lipomyces kononenkoae |
140000 | - |
glucoamylase II, gel filtration | Lipomyces kononenkoae |
150000 | - |
2 * 150000, glucoamylase I, SDS-PAGE | Lipomyces kononenkoae |
230000 | - |
glucoamylase I, gel filtration | Lipomyces kononenkoae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lipomyces kononenkoae | - |
CBS 5608 mutant | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | glucoamylase I contains 7.5% carbohydrate, glucoamylase II contains 6.5% carbohydrate | Lipomyces kononenkoae |
Purification (Comment) | Organism |
---|---|
glucoamylase I and II | Lipomyces kononenkoae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-cyclodextrin + H2O | - |
Lipomyces kononenkoae | glucose + ? | - |
? | |
beta-cyclodextrin + H2O | - |
Lipomyces kononenkoae | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 150000, glucoamylase I, SDS-PAGE | Lipomyces kononenkoae |
monomer | 1 * 128000, glucoamylase II, SDS-PAGE | Lipomyces kononenkoae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Lipomyces kononenkoae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 60 | stable | Lipomyces kononenkoae |
70 | - |
rapid inactivation above | Lipomyces kononenkoae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
- |
Lipomyces kononenkoae |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 6.5 | stable | Lipomyces kononenkoae |
5 | 8 | stable | Lipomyces kononenkoae |