Application | Comment | Organism |
---|---|---|
synthesis | the immobilized enzyme with high operational stability can be used for continuous production of glucose from soluble dextrin | Aspergillus niger |
General Stability | Organism |
---|---|
high operational stability of the enzyme immobilized onto poly(2-hydroxyethyl methacrylate)/ethylene glycol dimetharylate microspheres | Aspergillus niger |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.17 | - |
Dextrin | free enzyme | Aspergillus niger | |
0.83 | - |
Dextrin | immobilized enzyme | Aspergillus niger | |
6.2 | - |
maltose | free enzyme | Aspergillus niger | |
27.4 | - |
maltose | immobilized enzyme | Aspergillus niger |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dextrin + H2O | - |
Aspergillus niger | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
free enzyme | Aspergillus niger |
55 | - |
immobilized enzyme | Aspergillus niger |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 60 | 30°C: about 30% of maximal activity, immobilized enzyme, 30°C: about 50% of maximal activity, free enzyme, 60°C: about 50% of maximal activity, free enzyme, 60°C: about 90% of maximal activity, immobilized enzyme | Aspergillus niger |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
thermostability of immobilized glucoamylase increases considerably as a result of covalent immobilization onto poly(2-hydroxyethyl methacrylate)/ethylene glycol dimetharylate microspheres | Aspergillus niger |
70 | - |
250 min, immobilized enzyme loses 60% of its activity, half-life: 220 min. Free enzyme loses 75% of its activity, half-life 140 min | Aspergillus niger |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
2.5 | 8 | pH 2.5: about 35% of maximal activity, immobilized enzyme, pH 2.5: about 55% of maximal activity, free enzyme, pH 8.0: about 25% of maximal activity, free enzyme, pH 8.0: about 50% of maximal activity, immobilized enzyme | Aspergillus niger |