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Literature summary for 3.2.1.3 extracted from

  • Yamasaki, Y.; Tsuboi, A.; Suzuki, Y.
    Two forms of glucoamylase from Mucor rouxianus. II. Properties of the two glucoamylases (1977), Agric. Biol. Chem., 41, 2139-2148.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
Hg2+
-
Mucor rouxianus
Pb2+
-
Mucor rouxianus
Tris
-
Mucor rouxianus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information 0.741 mg/ml, hydrolysis of amylopectin with glucoamylase I. 0.667 mg/ml, hydrolysis of amylopectin with glucoamylase II. 0.455 mg/ml. Hydrolysis of amylose with glucoamylase I. 0.286 mg/ml, hydrolysis of amylose with glucoamylase II. 1.11 mg/ml, hydrolysis of glycogen with glucoamylase I. 1.54 mg/ml, hydrolysis of glycogen with glucoamylase II Mucor rouxianus

Organism

Organism UniProt Comment Textmining
Mucor rouxianus
-
2 enzyme forms: glucoamylase I and II
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein 2.74% neutral sugar Mucor rouxianus

Source Tissue

Source Tissue Comment Organism Textmining
cell culture wheat bran culture Mucor rouxianus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amylopectin + H2O
-
Mucor rouxianus D-glucose + ?
-
?
amylose + H2O
-
Mucor rouxianus glucose + ? beta-glucose ?
glycogen + H2O
-
Mucor rouxianus glucose + ?
-
?
maltose + H2O
-
Mucor rouxianus 2 glucose
-
?
maltotriose + H2O
-
Mucor rouxianus maltose + glucose
-
?
phenyl alpha-maltoside + H2O
-
Mucor rouxianus glucose + phenyl alpha-glucoside
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
Mucor rouxianus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 60 40°C: about 65% of maximal activity, 60°C: about 50% of maximal activity, glucoamylase II, about 60% of maximal activity, amylase I Mucor rouxianus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
pH 4.6, 15 min, stable up to Mucor rouxianus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.6
-
glucoamylase I Mucor rouxianus
5
-
glucoamylase II Mucor rouxianus

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 8 30°C, 24 h, glucoamylase I, stable Mucor rouxianus
4 7.5 30°C, 24 h, glucoamylase II is stable Mucor rouxianus