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Literature summary for 3.2.1.3 extracted from
- Purification and characterization of the extracellular glucoamylase from the yeast Trichosporon adeninovorans (1987), J. Basic Microbiol., 27, 299-308.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| cellobiose | 5 mM, slight inhibition of starch hydrolysis | Blastobotrys adeninivorans |  |
| fructose | 5 mM, slight inhibition of starch hydrolysis | Blastobotrys adeninivorans | |
| sorbitol | 5 mM, slight inhibition of starch hydrolysis | Blastobotrys adeninivorans | |
| xylose | 5 mM, slight inhibition of starch hydrolysis | Blastobotrys adeninivorans | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | 4 g/l for maltose | Blastobotrys adeninivorans | |
| additional information | - |
additional information | 1.2 g/l for soluble starch | Blastobotrys adeninivorans |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Blastobotrys adeninivorans | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 225000 | - |
gel filtration | Blastobotrys adeninivorans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Blastobotrys adeninivorans | - |
LS3 | - |
| Blastobotrys adeninivorans LS3 | - |
LS3 | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Blastobotrys adeninivorans |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Blastobotrys adeninivorans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Blastobotrys adeninivorans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glycogen + H2O | - |
Blastobotrys adeninivorans | glucose + ? | - |
? | |
| glycogen + H2O | - |
Blastobotrys adeninivorans LS3 | glucose + ? | - |
? | |
| maltose + H2O | - |
Blastobotrys adeninivorans | 2 glucose | - |
? | |
| maltose + H2O | - |
Blastobotrys adeninivorans LS3 | 2 glucose | - |
? | |
| starch + H2O | soluble | Blastobotrys adeninivorans | glucose + ? | - |
? | |
| starch + H2O | wheat starch, potato starch, corn starch | Blastobotrys adeninivorans | glucose + ? | - |
? | |
| starch + H2O | soluble | Blastobotrys adeninivorans LS3 | glucose + ? | - |
? | |
| starch + H2O | wheat starch, potato starch, corn starch | Blastobotrys adeninivorans LS3 | glucose + ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | 70 | in dependence of substrate concentration | Blastobotrys adeninivorans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
15 min, stable in presence of 1.25% soluble starch, about 80% loss of activity without starch | Blastobotrys adeninivorans |
| 70 | - |
fairly stable at approximately 4% of soluble starch | Blastobotrys adeninivorans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 5.5 | - |
Blastobotrys adeninivorans |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | 6 | pH 3.5: about 50% of maximal activity, pH 6.0: about 35% of maximal activity | Blastobotrys adeninivorans |
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