Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.28 extracted from

  • Cardello, L.; Terenzi, H. F.; Jorge, J. A.
    A cytosolic trehalase from the thermophilic fungus Humicola grisea var. thermoidea (1994), Microbiology, 140, 1671-1677.
No PubMed abstract available
Search for more literature for 3.2.1.28

Inhibitors

Inhibitors Comment Organism Structure
ADP 10 mM, 51% inhibition Trichocladium griseum
AlCl3 1 mM, complete inhibition Trichocladium griseum
AMP 10 mM, 5% inhibition Trichocladium griseum
ATP 10 mM, 67% inhibition Trichocladium griseum
cellobiose 10 mM, 54% inhibition. Inhibitory effect is abolished by Ca2+ Trichocladium griseum
CuSO4 1 mM, 80% loss of activity Trichocladium griseum
fructose 10 mM, 45% inhibition. Inhibitory effect is abolished by Ca2+ Trichocladium griseum
lactose 10 mM, 68% inhibition. Inhibitory effect is abolished by Ca2+ Trichocladium griseum
maltose 10 mM, 54% inhibition. Inhibitory effect is abolished by Ca2+ Trichocladium griseum
sucrose 10 mM, 54% inhibition. Inhibitory effect is abolished by Ca2+ Trichocladium griseum
ZnCl2 1 mM, 10% loss of activity Trichocladium griseum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.86
-
 alpha,alpha-trehalose pH 5.5, 60°C Trichocladium griseum

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Trichocladium griseum 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
BaCl2 1 mM, 1.1fold activation Trichocladium griseum
CaCl2 5 mM, 2.9fold activation Trichocladium griseum
CoCl2 1 mM, 1.7fold activation Trichocladium griseum
MgCl2 10 mM, 1.2fold activation Trichocladium griseum
MnCl2 7.5 mM, 4.2fold activation Trichocladium griseum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
12000
-
 3 * 12000, SDS-PAGE Trichocladium griseum
360000
-
 gel filtration Trichocladium griseum

Organism

Organism UniProt Comment Textmining
Trichocladium griseum
-
 var. therrnoidea
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein contains 12% carbohydrate Trichocladium griseum

Purification (Commentary)

Purification (Comment) Organism
-
 Trichocladium griseum

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
 Trichocladium griseum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
106.7
-
 pH 5.5, 60°C, substrate: alpha,alpha-trehalose Trichocladium griseum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha,alpha-trehalose + H2O
-
 Trichocladium griseum beta-D-glucose + alpha-D-glucose
-
 ?

Subunits

Subunits Comment Organism
trimer 3 * 12000, SDS-PAGE Trichocladium griseum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Trichocladium griseum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Trichocladium griseum

pI Value

Organism Comment pI Value Maximum pI Value
Trichocladium griseum electrofocusing
-
 4