Cloned (Comment) | Organism |
---|---|
gene treH is organized in a gene cluster with the other two genes of the trehalose metabolism pathway, treZ and treY encoding malto-oligosyltrehalose trehalohydrolase and maltooligosyltrehalose synthase, DNA and amino acid sequence determination and analysis, overview. Expression of the N-terminally His-tagged active enzyme in Escherichia coli | Nostoc punctiforme |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CaCl2 | over 90% inhibition at 10 mM | Nostoc punctiforme | |
MgCl2 | over 90% inhibition at 10 mM | Nostoc punctiforme | |
MgSO4 | over 90% inhibition at 20 mM | Nostoc punctiforme | |
MnCl2 | over 90% inhibition at 10 mM | Nostoc punctiforme | |
additional information | the enzyme activity is reduced under water-stress | Nostoc punctiforme | |
NaCl | strong inhibition at 10 mM, treatment of cells leads to accumulation of trehalose | Nostoc punctiforme | |
Tris | almost complete inhibition at 5 mM | Nostoc punctiforme |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
18 | - |
trehalose | pH 7.5, 37°C, recombinant enzyme | Nostoc punctiforme |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52000 | - |
x * 52000, recombinant His-tagged active enzyme, SDS-PAGE | Nostoc punctiforme |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Nostoc punctiforme | mechanism of trehalose accumulation in response to desiccation and salt stress, overview | ? | - |
? | |
additional information | Nostoc punctiforme IAM M-15 | mechanism of trehalose accumulation in response to desiccation and salt stress, overview | ? | - |
? | |
trehalose + H2O | Nostoc punctiforme | - |
2 D-glucose | - |
? | |
trehalose + H2O | Nostoc punctiforme IAM M-15 | - |
2 D-glucose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nostoc punctiforme | B6ZIV1 | gene treH | - |
Nostoc punctiforme IAM M-15 | B6ZIV1 | gene treH | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme by His affinity chromatography to homogeneity | Nostoc punctiforme |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
determination of trehalose contents in differently treated cells, overview | Nostoc punctiforme |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | mechanism of trehalose accumulation in response to desiccation and salt stress, overview | Nostoc punctiforme | ? | - |
? | |
additional information | mechanism of trehalose accumulation in response to desiccation and salt stress, overview | Nostoc punctiforme IAM M-15 | ? | - |
? | |
trehalose + H2O | - |
Nostoc punctiforme | 2 D-glucose | - |
? | |
trehalose + H2O | - |
Nostoc punctiforme IAM M-15 | 2 D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 52000, recombinant His-tagged active enzyme, SDS-PAGE | Nostoc punctiforme |
Synonyms | Comment | Organism |
---|---|---|
trehalase | - |
Nostoc punctiforme |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Nostoc punctiforme |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Nostoc punctiforme |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme catalyzes the last step in the trehalose metabolism and is the key enzyme controlling the pathway in response to water conditions | Nostoc punctiforme |