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Literature summary for 3.2.1.28 extracted from
- Purification and properties of an extracellular conidial trehalase from Humicola grisea var. thermoidea (1990), Biochim. Biophys. Acta, 1036, 41-46.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | Ca2+ abolishes inhibitory effect | Trichocladium griseum |  |
| AMP | Ca2+ abolishes inhibitory effect | Trichocladium griseum | |
| ATP | - |
Trichocladium griseum | |
| phosphate | Ca2+ abolishes inhibitory effect | Trichocladium griseum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.3 | - |
trehalose | - |
Trichocladium griseum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Trichocladium griseum | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activates | Trichocladium griseum | |
| Co2+ | activates | Trichocladium griseum | |
| Mn2+ | activates | Trichocladium griseum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 580000 | - |
gel filtration | Trichocladium griseum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| trehalose + H2O | Trichocladium griseum | - |
D-glucose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trichocladium griseum | - |
var. thermoidea | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | contains 56% carbohydrate | Trichocladium griseum |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Trichocladium griseum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| conidium | - |
Trichocladium griseum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 73.9 | - |
- |
Trichocladium griseum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| trehalose + H2O | - |
Trichocladium griseum | D-glucose | - |
? | |
| trehalose + H2O | absolute specificity | Trichocladium griseum | D-glucose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | polypeptide bands of 84000 Da, 98000 Da and 105000 Da are detected on SDS-PAGE | Trichocladium griseum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Trichocladium griseum |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 75 | about 35% of maximal activity at 40°C and at 75°C | Trichocladium griseum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.6 | - |
sodium acetate buffer | Trichocladium griseum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 7.5 | pH 4.0: about 35% of maximal activity, pH 7.5: about 30% of maximal activity | Trichocladium griseum |
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