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Literature summary for 3.2.1.28 extracted from

  • Nakano, M.; Sacktor, B.
    Isolation and characterization of four forms of trehalase from rabbit kidney cortex (1985), J. Biochem., 97, 1329-1335.
    View publication on PubMed
Search for more literature for 3.2.1.28

General Stability

General Stability Organism
enzyme form A1 becomes labile on addition of glycerol, activity in presence of 20% or 40% glycerol is about 30-40% lower than in absence of glycerol. Enzyme form B2 becomes more stable on addition of glycerol, activity in presence of 20% or 40% glycerol is about 30 higher than in absence of glycerol Oryctolagus cuniculus

Inhibitors

Inhibitors Comment Organism Structure
Hg2+ the glycoprotein enzyme forms are more susceptible than the nonglycoprotein forms Oryctolagus cuniculus
methyl beta-glucoside
-
 Oryctolagus cuniculus
phlorizin
-
 Oryctolagus cuniculus
Tris
-
 Oryctolagus cuniculus

Metals/Ions

Metals/Ions Comment Organism Structure
Na+ the glycoprotein enzyme forms show lower activation than the nonglycoprotein forms Oryctolagus cuniculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
175000
-
 gel filtration Oryctolagus cuniculus

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus
-
 2 nonglycoprotein enzyme forms: A1 and B1 and 2 glycoprotein enzyme form A2 and B2
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein 2 nonglycoprotein enzyme forms: A1 and B1, and 2 glycoprotein enzyme forms: A2 and B2 Oryctolagus cuniculus

Purification (Commentary)

Purification (Comment) Organism
4 enzyme form Oryctolagus cuniculus

Source Tissue

Source Tissue Comment Organism Textmining
kidney cortex Oryctolagus cuniculus
-

Storage Stability

Storage Stability Organism
-80°C, 9 days, 15% loss of activity of enzyme form A1, 5% loss of activity of enzyme form A2, 35% loss of activity of enzyme form B1, 65% loss of activity of enzyme form B2 Oryctolagus cuniculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
trehalose + H2O
-
 Oryctolagus cuniculus D-glucose
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.8 6 phosphate buffer Oryctolagus cuniculus